1887

Abstract

SUMMARY

A lethal mixture of the three factors (I, II, III) of anthrax toxin had none of the following enzyme activities: adenosine triphosphatase, alkaline or acid phosphatase, catalase, collagenase, ribonuclease, desoxyribonuclease, gelatinase, hyaluronidase, lecithinase, lipase, proteinase. The mode of action of anthrax toxin is unknown. Although factor II is the only factor which is immunogenic when injected alone, the addition of factor I results in an increased immunizing activity; however, the activity of this mixture can be decreased by adding factor III. The possible effect of these results on preparations used to immunize against anthrax is discussed.

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/content/journal/micro/10.1099/00221287-31-2-329
1963-05-01
2021-08-04
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References

  1. Alburn H. E., Whitley R. W. 1951; Factors affecting the assay of hyaluronidase. J. Mol. Chem 192:379
    [Google Scholar]
  2. Belton F. C., Strange R. E. 1954; Studies on a protective antigen produce. in vitro from Bacillus anthracis; medium and methods of production. Brit. J. exp. Path 35:144
    [Google Scholar]
  3. Bier M. 1955; Lipases. Meth. Enzymol 1:627
    [Google Scholar]
  4. Harris-Smith P. W., Smith H., Keppie J. 1958; Productio. in vitro of the toxin of Bacillus anthracis previously recognized in vivo. J. gen. Microbiol 19:91
    [Google Scholar]
  5. Hechter O. 1947; Studies on spreading factors. I. The importance of mechanical factors in hyaluronidase action in skin. J. exp. Med 85:77
    [Google Scholar]
  6. Keppie J., Cocking E. C, Witt K., Smith H. 1960; The chemical basis of the virulence o. Pasteurella pestis. III. An immunogenic product obtained from Past, pestis which protects both guinea pigs and mice. Brit. J. exp. Path 41:577
    [Google Scholar]
  7. Kielley W. W., Meyerhof O. 1950; Studies on adenosinetriphosphatase of muscle. III. The lipoprotein nature of the magnesium-activated adenosinetriphosphatase. J. biol. Chem 183:391
    [Google Scholar]
  8. King E. J. 1951 Microanalysis in Medical Biochemistry69 London: J. and A. Churchill Ltd;
  9. Kunitz M. 1946; A spectrophotometric method for the measurement of ribonuclease activity. J. biol. Chem 164:563
    [Google Scholar]
  10. Kunitz M. 1947; Crystalline soya-bean trypsin inhibitor. II. General properties. J. gen. Physiol 30:291
    [Google Scholar]
  11. Kunitz M. 1950; Crystalline desoxyribonuclease. I. Isolation and general properties. Spectrophotometric method for the measurement of desoxyribonuclease activity. J. gen. Physiol 33:349
    [Google Scholar]
  12. Macfarlane M. G., Knight B. C. J. G. 1941; The biochemistry of bacterial toxins. I. The lecithinase activity o. Clostridium welchii toxins. Biochem. J 35:884
    [Google Scholar]
  13. Miles A. A., Wilhelm D. L. 1955; Enzyme-like globulins from serum reproducing the vascular phenomena of inflammation. I. An activable permeability factor and its inhibitor in guinea-pig serum. Brit. J. exp. Path 36:71
    [Google Scholar]
  14. Oakley C. L., Warrack G. H., van Heyningen W. E. 1946; The collagenase (k toxin) of Clostridium welchii type A. J. Path. Bact 58:229
    [Google Scholar]
  15. Sargeant K., Stanley J. L., Smith H. 1960; The serological relationship between purified preparations of factors I and II of the anthrax toxin produce. in vivo in vitro. J. gen. Microbiol 22:219
    [Google Scholar]
  16. Smith H., Gallop R. C. 1956; The chemical basis of the virulence o. Bacillus anthracis. VI. An extracellular immunising aggressin isolated from exudates of infected guinea pigs. Brit. J. exp. Path 37:144
    [Google Scholar]
  17. Smith H., Keppie J., Pearce J. H., Witt K. 1962; The chemical basis of the virulence o. Brucella abortus. IV. Immunogenic products from Brucella abortus grown in vivo and in vitro. Brit. J. exp. Path 43:538
    [Google Scholar]
  18. Smith H., Keppie J., Stanley J. L. 1955; The chemical basis of the virulence o. Bacillus anthracis. V. The specific toxin produced by B. anthracis in vivo. Brit. J. exp. Path 36:460
    [Google Scholar]
  19. Smith H., Stanley J. L. 1962; Purification of the third factor of the anthrax toxin. J. gen. Microbiol 29:517
    [Google Scholar]
  20. Smith H., Tempest D. W., Stanley J. L., Harris-Smith P. W., Gallop R. C. 1956; The chemical basis of the virulence o. Bacillus anthracis. VII. Two components of the anthrax toxin ; their relationship to known immunising aggressins. Brit. J. exp. Path 37:263
    [Google Scholar]
  21. Stanley J. L., Sargeant K., Smith H. 1960; Purification of factors I and II of the anthrax toxin produce. in vivo. J. gen. Microbiol 22:206
    [Google Scholar]
  22. Stanley J. L., Smith H. 1961; Purification of factor I and recognition of a third factor of the anthrax toxin. J. gen. Microbiol 26:49
    [Google Scholar]
  23. Strange R. E., Thorne C. B. 1958; Further purification studies on the protective antigen o. Bacillus anthracis produced in vitro. J. Bact 76:192
    [Google Scholar]
  24. Thorne C. B., Molnar D. M., Strange R. E. 1960; Production of toxi. in vitro by Bacillus anthracis and its separation into two components. J. Bact 79:250
    [Google Scholar]
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