1887

Microbiology Society logo

Volume 31, Issue 2

Mutants of Aerobacter aerogenes Blocked in the Accumulation of Inorganic Polyphosphate Free

Abstract

SUMMARY

Mutants of blocked in the accumulation of inorganic polyphosphate were isolated by the following method. Organisms previously subjected to ultraviolet (u.v.) irradiation were induced to accumulate polyphosphate in medium containing P. Upon subsequent growth in medium devoid of phosphate, RNA and DNA synthesis took place at the expense of polyphosphate. The organisms containing radioactive DNA were inactivated by decay of the P during prolonged cold storage. From the survivors, mutants unable to form volutin granules were selected by microscopic examination. The mutants fell into two classes. The majority did not accumulate polyphosphate when phosphate was added to a phosphate-starved culture, but did so upon prolonged sulphur starvation. The remainder of the mutants did not accumulate polyphosphate under either condition. Both kinds of mutant grew well in a defined medium and showed no obvious physiological disabilities.

  • Received:
  • Published Online:
© Society for General Microbiology, 1962
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-2-241
1963-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/2/mic-31-2-241.html?itemId=/content/journal/micro/10.1099/00221287-31-2-241&mimeType=html&fmt=ahah

References

  1. Fuerst C. R., Stent G. S. 1956; Inactivation of bacteria by decay of incorporated radioactive phosphorus. J. gen. Physiol 40:73
     [Google Scholar]
  2. Harold F. M. 1960; Accumulation of inorganic polyphosphate in mutants o. Neurospora crassa. Biochim. biophys. Acta 45:172
     [Google Scholar]
  3. Harold F. M. 1962; Depletion and replenishment of the inorganic polyphosphate pool i. Neurospora crassa. J. Bact 83:1047
     [Google Scholar]
  4. Hoffmann-Ostenhof O., Weigekt W. 1952; Über die mögliche Funktion des polymeren Metaphosphats als Speicher energiereichen Phosphats in der Hefe. Naturwissenschaften 38:303
     [Google Scholar]
  5. Katchman B. J., Fetty W. O. 1955; Phosphorus metabolism in growing cultures o. Saccharomyces cerevisiae. J. Bact 69:607
     [Google Scholar]
  6. Kuhl A. 1960; Die Biologie der kondensierten anorganischen Phosphate. Ergebn. Biol 23:144
     [Google Scholar]
  7. Laybourn R. L. 1924; A modification of Albert’s stain for the diphtheria bacillus. J. Amer. med. Ass 83:121
     [Google Scholar]
  8. Sall T., Mudd S., Takagi A. 1958; Phosphate accumulation and utilization as related to synchronized cell division o. Corynebacterium diphtheriae. J. Bact 76:640
     [Google Scholar]
  9. Scherbaum O. H. 1960; Synchronous division of microorganisms. Annu. Rev. Microbiol 14:283
     [Google Scholar]
  10. Smith I. W., Wilkinson J. F., Duguid J. P. 1954; Volutin production i. Aerobacter aerogenes due to nutrient imbalance. J. Bact 68:450
     [Google Scholar]
  11. Wiame J. M. 1949; The occurrence and physiological behavior of two metaphosphate fractions in yeast. J. biol. Chem 178:919
     [Google Scholar]
  12. Widra A. 1959; Metachromatic granules of microorganisms. J. Bact 78:664
     [Google Scholar]
  13. Wilkinson J. F., Duguid J. P., Edmonds P. N. 1954; The distribution of polysaccharide production in Aerobacter and Escherichia strains and its relation to antigenic character. J. gen. Microbiol 11:59
     [Google Scholar]
  14. Wilkinson J. F., Duguid J. P. 1960; The influence of cultural conditions on bacterial cytology. Int. Rev. Cytol. 9:1
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-2-241
Loading
Mutants of Aerobacter aerogenes Blocked in the Accumulation of Inorganic Polyphosphate
Microbiology 31, 241 (1963); https://doi.org/10.1099/00221287-31-2-241
/content/journal/micro/10.1099/00221287-31-2-241
/content/journal/micro/10.1099/00221287-31-2-241
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error