1887

Abstract

SUMMARY

Foot-and-mouth disease (FMD) virus was partially inactivated by several methods: incubation with dilute formaldehyde or acetylethylene-imine, ultraviolet (U.V.) irradiation, heating, or by mixing with type specific antiserum, trypsin or extracts from cells susceptible to the virus. The serological properties of the treated virus preparations were studied by complement-fixation and agar diffusion tests and their content of infective ribonucleic acid (RNA) determined by phenol extraction. The ability of the treated preparations to attach to susceptible cultivated pig kidney cells was examined. The decrease in viral infectivity when FMD virus was treated with formaldehyde or acetylethyleneimine, U.V., or heat at 25° or 37° was proportional to the loss of infective RNA, with little impairment of its serological properties or its ability to attach to susceptible cells. In contrast, loss of viral infectivity on mixing with antiserum, trypsin or cell extracts was due to the failure of the virus to attach to susceptible cells; the viral RNA is still present in an infective form in these mixtures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-2-179
1963-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/2/mic-31-2-179.html?itemId=/content/journal/micro/10.1099/00221287-31-2-179&mimeType=html&fmt=ahah

References

  1. Bachrach H. L. 1961; Thermal degradation of foot-and-mouth disease virus into infectious ribonucleic acid. Proc. Soc. exp. Biol., N.Y 107:610
    [Google Scholar]
  2. Brooksby J. B. 1952; The technique of complement fixation in foot-and-mouth disease research. A.R.C. Rep. Ser12
  3. Brown F., Cartwright B. 1960; Purification of the virus of foot-and-mouth disease by fluorocarbon treatment and its dissociation from neutralizing antibody. J. Immunol 85:309
    [Google Scholar]
  4. Brown F., Cartwright B. 1961; Dissociation of foot-and-mouth disease virus into its nucleic acid and protein components. Nature, Lond 192:1163
    [Google Scholar]
  5. Brown F., Cartwright B., Stewart D. L. 1961; Mechanism of infection of pig kidney cells by foot-and-mouth disease virus. Biochim. biophys. Acta 47:172
    [Google Scholar]
  6. Brown F., Cartwright B., Stewart D. L. 1962; Further studies on the infection of pig kidney cells by foot-and-mouth disease virus. Biochim. biophys. Acta 55:768
    [Google Scholar]
  7. Brown F., Crick J. 1958; Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. Virology 5:133
    [Google Scholar]
  8. Brown F., Sellers R. F., Stewart D. L. 1958; Infectivity of ribonucleic acid from mice and tissue culture infected with the virus of foot-and-mouth disease. Nature, Lond 182:535
    [Google Scholar]
  9. Brown F., Stewart D. L. 1959; Studies with infective ribonucleic acid from tissues and cell cultures infected with the virus of foot-and-mouth disease. Virology, 7:408
    [Google Scholar]
  10. Gierer A., Schramm G. 1956; Infectivity of ribonucleic acid from tobacco mosaic virus. Nature, Lond 177:702
    [Google Scholar]
  11. Martin W. B., Chapman W. G. 1961; The tissue culture test for assaying the virus and neutralizing antibody of foot-and-mouth disease and its application to the measurement of immunity in cattle. Res. vet. Sci 2:53
    [Google Scholar]
  12. Nakamura M. 1961; A comparison of the yields of infectious ribonucleic acid from heated and ultraviolet irradiated mouse encephalomyelitis virus (GD VII strain). J. Immunol 87:530
    [Google Scholar]
  13. Sellers R. F. 1955; Growth and titration of the viruses of foot-and-mouth disease and vesicular stomatitis in kidney monolayer tissue cultures. Nature, Lond 176:547
    [Google Scholar]
  14. Skinner H. H. 1951; Propagation of strains of foot-and-mouth disease virus in un-weaned white mice. Proc. R. Soc. Med 44:1041
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-2-179
Loading
/content/journal/micro/10.1099/00221287-31-2-179
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error