Granular cultures of changed their growth habit when grown on a medium containing, as the source of nitrogen, the 19 L-amino acids found in hydrolysed casein, a response not seen when methionine was omitted. In contrast, pleomorphic cultures of grown on amino acid medium were not modified. The methionine effect has been used successfully to differentiate between normal granular cultures mutant pleomorphic cultures. Also, isolates characterized as atypical pleomorphs could be classified as metabolically granular or pleomorphic.


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