Glucose and certain other sugars accelerated hydrogen sulphide production from l-cysteine by washed cells of Escherichia coli (strain Crookes) which had been grown in the presence of l-cysteine. On the other hand, glucose or some other sugars in protein hydrolysate media containing l-cysteine suppressed the synthesis of an enzyme(s) which mediates the formation of hydrogen sulphide from l-cysteine. Glucose accelerated sulphide formation from l-cysteine by sonicated preinduced cells, although activity was unstable to such treatment. Both effects of glucose were influenced by the amino acid content of the medium. Sulphide production probably resulted through the action of cysteine desulphydrase; certain evidence suggested that a transaminase linked to β-mercaptopyruvate desulphurase also may have functioned. Apparently glucose repressed the induction of one or more enzymes concerned with cysteine degradation.
ChristensenH. N.1955 Mode of transport of amino acids into cells. A Symposium on Amino Acid Metabolism63McElroyW. D., GlassH. B. Baltimore: The Johns Hopkins Press;
CohnM., HoribataK.1959; Physiology of the inhibition by glucose of the induced synthesis of the β-galactoside-enzyme system of Escherichia coli. J. Bact. 78:624
DesnuelleP., FromageotC.1939; La décomposition anaérobie de la cysteine par Bacterium coli. I. Existence d’une cystéinase, ferment d’adaptation. Enzymologia 6:80
GaleE. F., FolkesJ. P.1953; The assimilation of amino acids by bacteria. 14. Nucleic acid and protein synthesis in Staphylococcus aureus. Biochem. J. 53:483
GrossS. R.1959; Enzymatic autoinduction and the hypothesis of intracellular permeability barriers in Neurospora. Trans. N. Y. Acad. Science Ser. n 22:44
HansonJ., MantelE.1953; Untersuchungen über den intermediaren S-Stoffwechsel. IV. Über die im Substrat gelegenen Bedingungen der Desulfurierbarkeit durch Escherichia coli. Hoppe-Seyl. Z. 295:141
MagasanikB., MagasanikA. K., NeidhardtF. C.1959 Regulation of growth and composition of the bacterial cell. CIBA Foundation SymposiumRegulation of Cell Metabolism334WolstenholmeG. E. W., O’ConnorC. M. London: J. and A. Churchill Ltd;
SiekevitzP.1959 On the meaning of intracellular structure for metabolic regulation. CIBA Foundation SymposiumRegulation of Cell Metabolism17WolstenholmeG. E. W., Μ. O’ConnorC. London: J. & A. Churchill Ltd;
SingerT. P., KearneyE. B.1955 Enzymatic pathways in the degradation of sulfur-containing amino acids. A Symposium on Amino Acid Metabolism558McElroyW. D., GlassH. B. Baltimore: The Johns Hopkins Press;
SudaM., SaigoT., IchiharaA.1954; The action of l-cysteine desulfhydrase. I. l-cysteine desulfhydrase isolated from bacteria. Med. J. Osaka Univ. 5:127Chem. Abstr. 48:11508ab