1887

Abstract

SUMMARY:

Damage to living organisms by X-rays (but not by ultraviolet radiation) is generally enhanced by the presence of oxygen, which probably acts at a physico-chemical level. Sensitivity to both u.v.- and X-radiation may also be related to the presence of oxygen acting as a metabolic factor during the growth of micro-organisms before or after irradiation.

Anaerobic growth of strain B after u.v. or X-rays decreased lethal damage, the treatment being most effective after u.v.-and more effective after X-rays delivered in the absence than in the presence of oxygen. In this respect anaerobic growth corresponded with a variety of inhibitory conditions after irradiation, and rescue by anaerobic growth was about as effective as chloramphenicol treatment; but when these two treatments were used together, the effectiveness of each was decreased.

The effects of u.v.- and X-radiation on B/r were unchanged by anaerobic growth after irradiation; but anaerobic growth beforehand resulted in survival curves which were much more sigmoid in shape than those pertaining to aerobically grown organisms. This tendency was noted with all strains tested. Anaerobic growth before X-irradiation did not alter the effect of oxygen during irradiation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-30-2-307
1963-02-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/30/2/mic-30-2-307.html?itemId=/content/journal/micro/10.1099/00221287-30-2-307&mimeType=html&fmt=ahah

References

  1. Alper T. 1955; Bacteriophage as indicator in radiation chemistry. Radiet. Res 2:119
    [Google Scholar]
  2. Alper T. 1956; The modification of damage caused by primary ionization of biological targets. Radiet. Res 5:573
    [Google Scholar]
  3. Alper T. 1959; Variability of the oxygen effect observed with micro-organisms. Part I. Haploid yeast; single and budding cells. Int. J. rod. Biol 1:414
    [Google Scholar]
  4. Alper T. 1961; Variability of the oxygen effect observed with micro-organisms. Part II. Escherichia coli B. Int. J. rod. Biol 3:369
    [Google Scholar]
  5. Alper T. 1962; Evidence for two resolvable sites of action of radiation on microorganisms. Abh. dtsch. Akad. Wiss., Berl Klasse für Medizin 1153
    [Google Scholar]
  6. Alper T., Fowler J. F., Morgan R. L., Vonberg D. D., Ellis F., Oliver R. 1962; The characterization of the ‘Type C’ survival curve. Brit. J. Radiol 35:722
    [Google Scholar]
  7. Alper T., Gillies N. E. 1958; Restoration of Escherichia coli strain B after irradiation: its dependence on suboptional growth conditions. J. gen. Microbiol 18:461
    [Google Scholar]
  8. Alper T., Gillies N. E. 1960a; The relationship between growth and survival after irradiation of Escherichia coli strain B and two resistant mutants. J. gen. Microbiol 22:113
    [Google Scholar]
  9. Alper T., Gillies N. E. 1960b; The influence of metabolic events immediately after irradiation on the difference in sensitivity between E. coli B and E. coli B/r. ‘Immediate and low level effects of ionizing radiations Conference, Venice June 1959.’. Supplement to Int. J. rad. Biol1960305
    [Google Scholar]
  10. Alper T., Gillies N. E., Elkind M. M. 1960; The sigmoid survival curve in radio-biology. Nature, Lond 186:1062
    [Google Scholar]
  11. Batchelor A., Bewley D. K., Morrison R., Stevenson J. A. 1959; Electron Therapy at 8 MeV. Brit. J. Radiol 32:332
    [Google Scholar]
  12. Charles R. L., Zimmermann L. N. 1956; Dark reactivation in ultra-violet irradiated Escherichia coli. J. Bact 71:611
    [Google Scholar]
  13. Gillies N. E. 1961; The use of auxotrophic mutants to study restoration in E. coli B after ultraviolet irradiation. Int. J. rad. Biol 3:379
    [Google Scholar]
  14. Gillies N. E., Alper T. 1959; Reduction in the lethal effects of radiations on Escherichia coli B by treatment with chloramphenicol. Nature, Lond 183:237
    [Google Scholar]
  15. Hollaender A., Stapleton G. E., Martin F. L. 1951; X-ray sensitivity of E. coli as modified by oxygen tension. Nature, Lond 167:103
    [Google Scholar]
  16. Howard-Flanders P., Alper T. 1957; The sensitivity of micro-organisms to irradiation under controlled gas conditions. Radiet. Res. 7:518
    [Google Scholar]
  17. Roberts R. B., Aldous E. 1949; Recovery from ultraviolet irradiation in Escherichia coli. J. Bact 57:363
    [Google Scholar]
  18. Sargent T. 1961; Effects of pH and anoxia on the cell morphology and X-ray sensitivity of Escherichia coli. Radiet. Res 14:323
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-30-2-307
Loading
/content/journal/micro/10.1099/00221287-30-2-307
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error