1887

Abstract

SUMMARY:

None of the classes of major chemical constituents of the walls of bacteria and yeasts is exclusive to organisms which give a Gram-positive reaction. Walls of Gram-positive bacteria are richer in mucopeptide than are those of Gram-negative bacteria. The latter are characterized by high lipid contents. Yeast walls are rich in polysaccharide complexes. There appears to be a broad correlation between the Gram reaction and the decreased leakage of P compounds from labelled cells on exposure to increasing ethanol concentrations in the range 50--100% (v/v) ethanol. The data on the release of P compounds are in accord with the cell-wall ‘permeability’ mechanism for the Gram reaction and imply quantitative rather than all or none differences in Gram behaviour. Mechanical rupture of the cell wall or digestion of the wall with lysozyme rendered organisms which had been previously Gram stained in suspension by the technique of Wensinck & Boevé (1957), susceptible to decolorization with 96% (v/v) ethanol in water.

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1963-02-01
2021-07-28
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References

  1. Armstrong J. J., Baddiley J., Buchanan J. G., Carss B., Greenberg G. R. 1958; Isolation and structure of ribitol phosphate derivatives (teichoic acids) from bacterial cell walls. J. chem. Soc4344
    [Google Scholar]
  2. Bartholomew J. W., Cromwell T., Finkelstein H. 1959; Correlation between iodine permeability and the Gram characteristic of cells. Nature, Lond 183:123
    [Google Scholar]
  3. Bartholomew J. W., Mittwer T. 1952; The Gram stain. Bact. Rev 16:1
    [Google Scholar]
  4. Benians T. H. C. 1920; A further investigation into the principles underlying Gram’s stain, with special reference to the bacterial cell membrane. J. Path. Bact 23:401
    [Google Scholar]
  5. Burke V., Barnes M. W. 1929; The cell wall and the gram reaction. J. Bact 18:69
    [Google Scholar]
  6. Chelton E. T. J, Jones A. S. 1959; The Gram-staining reaction of disintegrated microorganisms. J. gen. Microbiol 21:652
    [Google Scholar]
  7. Churchman J. W., Siegel L. 1928; Cultural separation of bacteria on the basis of triphenylmethane coefficients. Stain Tech 3:73
    [Google Scholar]
  8. Deussen E. 1921; Die Gramsche Bakterienfärbung, ihr Wesen und ihre Bedeutung. Z. Hyg. Infektr.-Kr 93:512
    [Google Scholar]
  9. Dubos R. J., Macleod C. M. 1938; The effect of a tissue enzyme upon pneumococci. J. exp. Med 67:791
    [Google Scholar]
  10. Eisenberg P. 1910; Zur Theorie der Gramfestigkeit. Zbl. Bakt Abt. 1, Orig 56:193
    [Google Scholar]
  11. Falcone G., Nickerson W. J. 1956; Cell-wall mannan-protein of Baker’s yeast. Science 124:272
    [Google Scholar]
  12. Fischer R., Larose P. 1952; Contribution on the behaviour and structure of the cytoplasmic membrane of bacteria. Canad. J. med. Sci 30:86
    [Google Scholar]
  13. Gerhardt P., Vennes J. W., Britt Ε. M. 1956; Gram reaction of isolated protoplasts and surface membranes of Bacillus megaterium. J. Bact 72:721
    [Google Scholar]
  14. Gram C. 1884; Über die isolirte Färbung der Schizomyceten in Schnitt-und Trockenpräparaten. Fortschr. Med. 2:185
    [Google Scholar]
  15. Greenwood C. T. 1956; Aspects of the physical chemistry of starch. Advanc. Carbohyd. Chem 11:335
    [Google Scholar]
  16. Henry H., Stacey M. 1946; Histochemistry of the Gram staining reaction for microorganisms. Proc. Roy. Soc. B 133:391
    [Google Scholar]
  17. Henry H., Stacey M., Teece E. G. 1945; Nature of the gram positive complex in microorganisms. Nature, Lond 156:720
    [Google Scholar]
  18. Herbst E. J., Weaver R. H., Keister D. L. 1958; The Gram reaction and cell composition: diamines and polyamines. Arch. Biochem. Biophys 75:171
    [Google Scholar]
  19. Hucker G. J. 1921; A new modification and application of the Gram stain. J. Bact 6:395
    [Google Scholar]
  20. Jones A. S., Rizvi S. B. H., Stacey M. 1958; The phosphorus-containing compounds of Gram-positive and Gram-negative organisms in relation to the Gram staining reaction. J. gen. Microbiol 18:597
    [Google Scholar]
  21. Kaplan M. L., Kaplan L. 1933; The Gram stain and differential staining. J. Bact 25:309
    [Google Scholar]
  22. Kessler G., Nickerson W. J. 1959; Glucomannan-protein complexes from cell walls of yeasts. J. biol. Chem 234:2281
    [Google Scholar]
  23. Lamanna C., Mallette M. F. 1950; The relation of the Gram stain to the cell wall and the ribonucleic acid content of the cell. J. Bact 60:499
    [Google Scholar]
  24. Mitchell P., Moyle J. 1950; Occurrence of a phosphoric ester in certain bacteria: its relation to Gram staining and penicillin sensitivity. Nature, Lond 166:218
    [Google Scholar]
  25. Mitchell P., Moyle J. 1951; The glycerophospho-protein complex envelope of Micrococcus pyogenes. J. gen. Microbiol 5:981
    [Google Scholar]
  26. Mitchell P., Moyle J. 1954; The Gram reaction and cell composition: nucleic acids and other phosphate fractions. J. gen. Microbiol 10:533
    [Google Scholar]
  27. Mitchell P., Moyle J. 1957; Autolytic release and osmotic properties of ‘protoplasts’ from Staphylococcus aureus. J. gen. Microbiol 16:184
    [Google Scholar]
  28. Mitchell P., Moyle J. 1958; The positic acids of Staphylococcus aureus and other Gram-positive penicillin-sensitive bacteria: hydrolytic products and possible backbone structure. Proc. R. phys. Soc. Edinb 27:79
    [Google Scholar]
  29. Neide E. 1904; Die Alkoholentfärbung der nach Gram gefärben Bakterien als speciesdiagnose, in Verbindung mit einer Untersuchung der für die Gramfarbung in Betracht kommenden Faktoren. Zbl. Bakt Abt. 1, Orig. 35:508
    [Google Scholar]
  30. Northcote D. H., Hörne R. W. 1952; The chemical composition and structure of the yeast cell wall. Biochem. J 51:232
    [Google Scholar]
  31. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli. Publ. Carneg. Instn607
    [Google Scholar]
  32. Rondle C. J. M, Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  33. Salton M. R. J. 1951; The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J. gen. Microbiol 5:391
    [Google Scholar]
  34. Salton M. R. J. 1953a; The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim, biophys. Acta 10:512
    [Google Scholar]
  35. Salton M. R. J. 1953b; Cell structure and enzymic lysis of bacteria. J. gen. Microbiol 9:512
    [Google Scholar]
  36. Salton M. R. J. 1956; Bacterial cell walls. Bacterial Anatomy. Symp. Soc. gen. Microbiol 6:81
    [Google Scholar]
  37. Salton M. R. J. 1958; The lysis of micro-organisms by lysozyme and related enzymes. J. gen. Microbiol 18:481
    [Google Scholar]
  38. Salton M. R. J. 1961a; The anatomy of the bacterial surface. Bact. Rev 25:77
    [Google Scholar]
  39. Salton M. R. J. 1961b Microbial Cell Walls New York: John Wiley & Sons;
    [Google Scholar]
  40. Salton M. R. J, Horne R. W. 1951; Methods of preparation and some properties of cell walls. Biochim. biophys. Acta 7:177
    [Google Scholar]
  41. Salton M. R. J, Pavlik J. G. 1960; Wall composition and sensitivity to lysozyme. Biochim. biophys. Acta 39:398
    [Google Scholar]
  42. Salton M. R. J, Shafa F. 1958; Some changes in the surface structure of Gram-negative bacteria induced by penicillin action. Nature, Lond 181:1321
    [Google Scholar]
  43. Schumacher J. 1928; Über die chemische Zusammensetzung der lipoidsaüre und über künstlich grampositiv gemachte Hefezellen. Zbl. Bakt Abt. 1, Orig. 109:181
    [Google Scholar]
  44. Shugar D., Baranowska J. 1957; Gram staining of extracellular material. Biochim. biophys. Acta 23:227
    [Google Scholar]
  45. Shugar D., Baranowska J. 1958; Quantitative Gram-staining with labelled iodine. Nature, Lond 181:357
    [Google Scholar]
  46. Stacey M. 1949; The nature of the surface of Gram-positive bacteria. The Bacterial Surface. Symp. Soc. gen. Microbiol 1:29
    [Google Scholar]
  47. Stearn E. W., Stearn A. E. 1924; A new theory of the gram reaction. J. Bact 9:479
    [Google Scholar]
  48. Stearn E. W., Stearn A. E. 1930; The nature of the gram compound and its bearing on the mechanism of staining. J. Bact 20:287
    [Google Scholar]
  49. Webb M. 1948; The action of lysozyme on heat-killed gram-positive microorganisms. J. gen. Microbiol. 2:260
    [Google Scholar]
  50. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain B. J. gen. Microbiol 22:158
    [Google Scholar]
  51. Wensinck F., Boevé J. J. 1957; Quantitative analysis of the Gram reaction. J. gen. Microbiol 17:401
    [Google Scholar]
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