1887

Abstract

SUMMARY:

The adsorption of a tryptophan-requiring (tryp) hydroxymethylcytosine (HMC) phage (Ox 6) to bacteria has been compared with that to kaolin. In a system free from monovalent cations other than tris (2-amino-2-hydroxymethylpropane-1, 3-diol) buffer, adsorption in both cases was temperature-dependent and required not only tryptophan but an optimum concentration of NaCl. The adsorption to kaolin came to an equilibrium which was determined by temperature since it depended on a preliminary activation of the phage, which process was temperature-dependent. Elution of phage adsorbed to kaolin took place to a greater or less degree on altering any one of the three requirements, NaCl, tryptophan or temperature. In experiments with bacteria no equilibrium was reached because adsorption was followed rapidly by inactivation; no elution was demonstrable.

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/content/journal/micro/10.1099/00221287-30-2-183
1963-02-01
2024-12-05
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References

  1. Adams M. H. 1959 Bacteriophages145146 London: Interscience Publishers Ltd;
    [Google Scholar]
  2. Anderson T. F. 1948; The activation of the bacterial virus T4 by l-tryptophan. J. Bact 55:637
    [Google Scholar]
  3. Fildes P., Kay D. 1957; Tryptophan as a bacteriophage adsorption factor. Brit. J. exp. Path 38:563
    [Google Scholar]
  4. Fildes P., Kay D. 1959; The function of tryptophan in the adsorption of a bacteriophage. Brit. J. exp. Path 40:71
    [Google Scholar]
  5. Kay D., Fildes P. 1962a; Hydroxymethyleytosine-containing and tryptophan-dependent bacteriophages isolated from city effluents. J. gen. Microbiol 27:143
    [Google Scholar]
  6. Kay D., Fildes P. 1962b; The thermal lability of tryptophan-dependent bacteriophages. J. gen. Microbiol 27:147
    [Google Scholar]
  7. Luria S. E. 1953 General Virology165 London: Chapman and Hall;
    [Google Scholar]
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