SUMMARY: The adsorption of a tryptophan-requiring (tryp) hydroxymethylcytosine (HMC) phage (Ox 6) to bacteria has been compared with that to kaolin. In a system free from monovalent cations other than tris (2-amino-2-hydroxymethylpropane-1, 3-diol) buffer, adsorption in both cases was temperature-dependent and required not only tryptophan but an optimum concentration of NaCl. The adsorption to kaolin came to an equilibrium which was determined by temperature since it depended on a preliminary activation of the phage, which process was temperature-dependent. Elution of phage adsorbed to kaolin took place to a greater or less degree on altering any one of the three requirements, NaCl, tryptophan or temperature. In experiments with bacteria no equilibrium was reached because adsorption was followed rapidly by inactivation; no elution was demonstrable.


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