SUMMARY: The general form of assay for streptomycin described by Brownlee, Delves, Dorman, Green, Grenfell, Johnson & Smith (1948), based on an 8 x 8 quasi-Latin square layout of cavities on a large agar-covered glass plate, in place of Petri dishes, can be adapted to penicillin. A new design, using the principle of double confounding, allows the estimation by the usual four-point assay, of seven (instead of three) unknown solutions per plate of 64 holes. In routine use this assay gives fiducial limits (P = 0.95) of about ±9.6%, including dilution errors.

The assay procedure previously described in this Journal (Brownlee et al. 1948) for streptomycin, is immediately adaptable to penicillin. For dealing with large numbers of fermenter samples in particular, we have found modifications desirable, which we describe in the present paper.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error