1887

Abstract

Summary: Some 300 strains of bacteria representing twenty genera were grown on an acid-hydrolysed casein medium. Using the paper chromatographic technique of Woiwod (1949) a preliminary survey was made of the amino-acid and polypeptide composition of the bacterial culture filtrates. It was proved that: () changes may occur in the filtrate chromatogram which have group or species significance; () bacteria with simple nutrient requirements, i.e. which utilize ammonia, do not affect the chromatogram in the initial growth stage; () whenever the chromatogram is affected serine is the first amino-acid to be metabolized; () Gram-positive bacteria eliminate the aspartic acid spot but leave the basic amino-acid group unaffected, and the reverse occurs with Gram-negative bacteria; () many bacteria synthesize ninhydrin-positive material, presumably polypeptide, the synthesis of a given kind of polypep-tide being sometimes associated with a particular group or species of bacteria. By a disintegrator technique it was demonstrated that bacteria store many free amino-acids inside the cell.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-3-2-319
1949-05-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/3/2/mic-3-2-319.html?itemId=/content/journal/micro/10.1099/00221287-3-2-319&mimeType=html&fmt=ahah

References

  1. Consden R., Gordon A.H., Martin A.J.P. 1944; Qualitative analysis of proteins: a partition chromatographic method using paper. Biochem. J. 41:224
    [Google Scholar]
  2. Elliot W.H., Gale E.F. 1948; Glutamine-synthesizing system of Staphylococcus aureus: its inhibition by crystal violet and methionine-sulphoxide. Nature,; Lond.: 161129
    [Google Scholar]
  3. Gale E.F. 1947; The assimilation of amino-acids by bacteria. 1. The passage of certain amino-acids across the cell wall and their concentration in the internal environjnent of Streptococcus faecalis. . J. gen. Microbiol. 1:53
    [Google Scholar]
  4. Polson A. 1948; New amino-acids in bacterial hydrolysates. Nature,; Lond.: 161351
    [Google Scholar]
  5. Taylor E. 1947; The assimilation of amino-acids by bacteria. 3. Concentration of free amino-acids in the internal environment of various bacteria and yeasts. J. gen. Microbiol. 1:86
    [Google Scholar]
  6. Woiwod A.J. 1949; A technique for examining large numbers of bacterial culture filtrates by partition chromatography. J. gen. Microbiol. 3:312
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-3-2-319
Loading
/content/journal/micro/10.1099/00221287-3-2-319
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error