1887

Abstract

SUMMARY: Details are given of the ‘auxanographic’ method for identifying growthfactor requirements of micro-organisms, and particularly of mutant strains differing from a parent strain in having additional growth-factor requirements. Cells are distributed in a nutritionally deficient agar medium which is allowed to set in a Petri dish. Possible required nutrients are then spotted on the agar surface, thus enabling the corresponding exacting mutants to grow at those localities. The technique is only a labour-saving device which can be extremely efficient in shortening the preliminary steps in the work. It can also be applied to other uses, such as the identification of competitive interactions between nutrilites.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-3-1-122
1949-01-01
2020-01-28
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-3-1-122
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error