SUMMARY: A sensitive method for the assay of mμg. amounts of megacin is described. Sensitive organisms adsorb megacin and the saturation concentration is similar to bactericidal concentrations. The death of megacintreated organisms is, however, not coincident with adsorption but can be delayed or prevented by incubation at low temperature. Post-adsorption death is in fact proportional to the incubation temperature. Bactericidal amounts of megacin transform protoplasts of sensitive organisms to empty spherical ghosts and also appear to disrupt the permeability barrier of whole organisms. Megacin does not, however, degrade isolated or intact cytoplasmic membranes.


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