1887

Abstract

SUMMARY: A sensitive method for the assay of μg. amounts of megacin is described. Sensitive organisms adsorb megacin and the saturation concentration is similar to bactericidal concentrations. The death of megacintreated organisms is, however, not coincident with adsorption but can be delayed or prevented by incubation at low temperature. Post-adsorption death is in fact proportional to the incubation temperature. Bactericidal amounts of megacin transform protoplasts of sensitive organisms to empty spherical ghosts and also appear to disrupt the permeability barrier of whole organisms. Megacin does not, however, degrade isolated or intact cytoplasmic membranes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-29-4-603
1962-12-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/29/4/mic-29-4-603.html?itemId=/content/journal/micro/10.1099/00221287-29-4-603&mimeType=html&fmt=ahah

References

  1. Duguid J. P. 1951; Demonstration of bacterial capsules and slime. J. Path. Bad 63:673
    [Google Scholar]
  2. Erlanger B. F., Goode L. 1954; Gramicidin S: relationship of cyclic structure to antibiotic activity. Nature, Lond 174:840
    [Google Scholar]
  3. Fredericq P. 1957; Colicins. Annu. Rev. Microbiol 11:7
    [Google Scholar]
  4. Gratia A. 1925; Sur un remarquable example d’antagonisme entre deux souches de Colibadlle. C.R. Soc. Biol Paris: 93140
    [Google Scholar]
  5. Gratia A. 1932; Antagonisme microbien et bacteriophagie. Ann. Inst. Pasteur 48:413
    [Google Scholar]
  6. Heppel L. 1957 In Methods in Enzymology vol. 3 p. 137 Ed Colowiek S. P., Kaplan N. O. New York: Academic Press Inc.;
    [Google Scholar]
  7. Holland I. B. 1961; The purification and properties of megacin, a bacteriocin from Bacillus megaterium. Biochem. J 78:641
    [Google Scholar]
  8. Ivánovics G., Alföldi L. 1954; A new antibacterial principle: megacine. Nature, Lond 174:465
    [Google Scholar]
  9. Ivánovics G., Alföldi L. 1955; Observations on lysogenesis in Bacillus megaterium and on megacine, the antibacterial principle of this bacillus species. Acta microbiol. Acad. Sci. hung 2:275
    [Google Scholar]
  10. Ivánovics G., Alföldi L. 1957; Bacteriocinogenesis in Bacillus megaterium. J. gen. Microbiol 16:522
    [Google Scholar]
  11. Ivánovics G., Alföldi L., Nagy E. 1959a; Masked provirus in Bacillus megaterium strains. Acta mrol. Acad. Sci., Czech 3:23
    [Google Scholar]
  12. Ivánovics G., Alföldi L., Nagy E. 1959b; Mode of action of megacin. J. gen. Microbiol 21:51
    [Google Scholar]
  13. Ivánovics G., Nagy E. 1958; Hereditary aberrancy in growth of some Bacillus megaterium strains. J. gen. Microbiol 19:407
    [Google Scholar]
  14. McQuillen K. 1956 In Bacterial Anatomy. Symp. Soc. gen. Microbiol 6:127
    [Google Scholar]
  15. Newton B. A. 1956; The properties and mode of action of the polymyxins. Bad. Rev. 20:14
    [Google Scholar]
  16. Ozeki H., Stocker B. A. D., De Margerie H. 1959; Production of colicine by single bacteria. Nature, Lond 184:337
    [Google Scholar]
  17. Salton M. R. J. 1957; The properties of lysozyme and its action on microorganisms. Bad. Rev 21:82
    [Google Scholar]
  18. Salton M. R. J., Horne R. W. 1951; Studies of the bacterial cell wall. II. Methods of preparation and some properties of the bacterial cell wall. Biochim. biophys. Acta 7:177
    [Google Scholar]
  19. Spiegelman S., Aronson A. I., Fitz-James P. C. 1958; Isolation and characterization of nuclear bodies from protoplasts of Bacillus me&aterium. J. Bact 75:102
    [Google Scholar]
  20. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bad 66:688
    [Google Scholar]
  21. Weibull C., Bergstrom L. 1958; The chemical nature of the cytoplasmic membrane and cell wall of Bacillus megaterium, strain M. Biochim. biophys. Acta 30:340
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-29-4-603
Loading
/content/journal/micro/10.1099/00221287-29-4-603
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error