1887

Abstract

SUMMARY: During the submerged batch cultivation of a riboflavin-producing strain of three phases were observed. The first phase was characterized by rapid growth of mycelium, rapid utilization and oxidation of glucose and a decrease of pH value caused by accumulation of pyruvic acid. Subsequently acetoin accumulated in the medium. Glucose was oxidized incompletely since only 1.8 μmole oxygen were consumed/ μmole glucose utilized. The end of this phase was marked by exhaustion of glucose and cessation of growth. The second phase began with sporulation and was characterized by rapid synthesis of cell-bound riboflavin. Simultaneously a rapid increase in catalase activity and decreases of pyruvate and acetoin were observed. This was accompanied by a marked decrease in Qo on glucose while the Qo on pyruvate, threonine or acetaldehyde increased to a maximum. Ammonia accumulated in the medium and alkaline pH values were reached. The third phase was characterized by autolysis of mycelium which led to the release of riboflavin and to a decrease of enzymic activities. A comparison of all important physiological parameters was made with two strains of of different riboflavin productivity. On the basis of correlation between riboflavin formation, catalase activity and respiration on acetaldehyde, an hypothesis is proposed to explain over-production of riboflavin by a shift from the initial eytochrome type of terminal respiration to the flavoprotein type which is, however, accompanied by over-production of the flavin prosthetic group.

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1962-11-01
2024-04-18
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References

  1. Ashwell G. 1957 In Methods of Enzymology vol. 3 p. 85 Ed. by Colowick S. R., Kaplan N. O. New York: Academic Press Inc.;
    [Google Scholar]
  2. Avi-Dor Y., Yaniv H. 1952; The activity of catalase in Pasteurella tularensis. J. Bad. 63 751
    [Google Scholar]
  3. Boyd W. L., Lichstein H. C. 1953; Effect of carbohydrates on aspartic acid de-aminase activity of bacteria. Proc. Soc. exp. Biol. N.Y.: 82 45
    [Google Scholar]
  4. Brown E. G., Goodwin T. W., Jones O. T. G. 1958; Studies on the biosynthesis of riboflavin. 4. Purine metabolism and riboflavin synthesis in Eremothecium ashbyi. Biochem. J. 68 40
    [Google Scholar]
  5. Brown E. G., Goodwin T. W., Pendlington S. 1955; Studies on the biosynthesis of riboflavin. 2. Further observations on nitrogen metabolism and flavinogenesis in Eremothecium ashbyi. Biochem. J. 61 37
    [Google Scholar]
  6. Brown F., Hall L. P. 1950; Separation of carboxylate ions on the paper chromato-gram. Nature, Lond. 166 66
    [Google Scholar]
  7. Cavallini D., Frontali N. 1954; Quantitative determination of keto-acids by paper partition chromatography. Biochim. biophys. Acta 13 439
    [Google Scholar]
  8. Dikanskaya E. M. 1953; Issledovaniya po fiziologii pitaniya Eremothecium ashbyi. Microbiology, Moscow 22 256
    [Google Scholar]
  9. Dikanskaya E. M. 1954; Obrazovanie riboflavina mikroskopitsheskim gribom Eremothecium ashbyi. Trud. Inst. Mikrobiol. Akad. Nauk. 3 35
    [Google Scholar]
  10. Dulaney E. L., Grutter F. H. 1950; The nutritional requirements of Eremothecium ashbyi. Guill. Mycologia 42 717
    [Google Scholar]
  11. Friedeman T. E. 1957 In Methods of Enzymology vol. 3 p. 414 Ed. by Colowick S. R., Kaplan N. O. New York: Academic Press Inc.;
    [Google Scholar]
  12. Goodwin T. W. 1959; Production and biosynthesis of riboflavin in microorganisms. In Progress in Industrial Microbiology vol. 1 p. 138 Ed. Hockenhull D. J. D. London: Heywood Co.;
    [Google Scholar]
  13. Goodwin T. W., Horton A. A. 1960; Studies in flavinogenesis. 6. The role of threo-nine in riboflavin biosynthesis in Eremothecium ashbyi. Biochem. J. 75 53
    [Google Scholar]
  14. Goodwin T. W., Jones O. T. G. 1956; Studies on the biosynthesis of riboflavin. 3. The utilization of 14C-labelled serine for riboflavin biosynthesis by Eremothecium ashbyi. Biochem. J. 64 9
    [Google Scholar]
  15. Goodwin T. W., Pendlington S. 1954; Riboflavin in Eremothecium ashbyi. Nitro¬gen metabolism and flavinogenesis in Eremothecium ashbyi. Biochem. J. 57 631
    [Google Scholar]
  16. Goodwin T. W., Treble D. H. 1958; The incorporation of 2-14C/acetylmethylcarbinol (acetoin) into ring A of riboflavin by Eremothecium ashbyi ; a new route for the biosynthesis of an aromatic ring. Biochem. J. 70 14 p.
    [Google Scholar]
  17. Hickey R. J. 1953; Some nutritional requirements for biosynthesis of riboflavin by Eremothecium ashbyi. J. Bad. 66 27
    [Google Scholar]
  18. Holló J., Szamel J., Nyeste L., Tegerdy R. 1956; Recherches sur la production de riboflavin a partir de residus agricoles. Industr. agric. aliment. Paris: 73 519
    [Google Scholar]
  19. Kapralek F. 1957; Z&konitosti v prubehu submersni kultivace Eremothecium ashbyi. Preslia 29 113
    [Google Scholar]
  20. Katagiri H., Imai K. 1961; Mechanism of biosynthesis of riboflavin and its compounds. Abstr. 5th int. Congr. Biochem. 14 49
    [Google Scholar]
  21. Klungøsyr L. 1954; The biosynthesis of riboflavin in Eremothecium ashbyi. Acta chem. scand. 8 1292
    [Google Scholar]
  22. Krámli A., Szabó A. 1956; Die Wirkung von Redoxsystemen auf den Stoffwechsel von Mikroorganismen. 4. Veranderung der Riboflavinproduktion in geschiittelten Kulturen von Eremothecium ashbyi. Acta biol. Acad. sci. hung. 6 197
    [Google Scholar]
  23. Krampitz L. O. 1957 In Methods of Enzymology vol. 3 p. 278 Ed. by Colowick S. R., Kaplan N. O. New York: Academic Press Inc.;
    [Google Scholar]
  24. Lenhoff H. M., Nicholas D. J. D., Kaplan N. O. 1956; Effects of oxygen, iron and molybdenum on routes of electrontransfer in Pseudomonesfluorescens. J. biol. Chem. 220 983
    [Google Scholar]
  25. Lowry H. O., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193 265
    [Google Scholar]
  26. MacLaren J. A. 1952; The effects of certain purines and pyrimidines upon the produc¬tion of riboflavin by Eremothecium ashbyi.. J. Bad. 63 233
    [Google Scholar]
  27. McNutt W. S. 1954; The direct contribution of adenine to the biogenesis of riboflavin by Eremothecium ashbyi. J. biol. Chem. 210 511
    [Google Scholar]
  28. Mickelson M. N. 1950; The metabolism of glucose by Ashby a gossypii. J. Bact. 59 659
    [Google Scholar]
  29. Nicholas D. J. D. 1956; Trace metal requirements and some enzyme systems in a ribo-flavine-requiring mutant of Neurospora crassa. J. gen. Microbiol. 15 470
    [Google Scholar]
  30. Pfeifer V. F., Tanner F. W., Traufler D. H. 1950; Riboflavin by fermentation with Ashbya gossypii. Indust. Engn Chem. (Industr.) 42 1776
    [Google Scholar]
  31. Plaut G. W. E. 1954a; Biosynthesis of riboflavin. 1. Incorporation of C14-labelled compounds into rings B and C. J. biol. Chem. 208 513
    [Google Scholar]
  32. Plaut G. W. E. 1954b; Biosynthesis of riboflavin. 2. Incorporation of C14-labelled compounds into ring A. J. biol. Chem. 211 111
    [Google Scholar]
  33. Schneider W. C., Potter V. R. 1943; The assay of animal tissues for respiratory enzymes. 2. Succinic dehydrogenase and cytochrome oxidase. J. biol. Chem. 149 217
    [Google Scholar]
  34. Schopfer W. H. 1944; La biotin, l’aneurin et le mesoinositol, facteurs de croissance pour Eremothecium ashbyi Guill. La biosynthese de la flavine. Helv. chim. Acta 27 1017
    [Google Scholar]
  35. Shimizu S., Ohara S., Minoura K. 1952; Riboflavin production by Eremothecium ashbyi. 32. Form of flavin produced during cultivation. J. ferment. Tech. Japan 30 13 Reported in Chem. Abst. 48: 3449
    [Google Scholar]
  36. Stárka J. 1957; Properties of Eremothecium ashbyi strain-producing riboflavin. J.gen. Microbiol. 17, iv.
    [Google Scholar]
  37. Sumner J. B. 1941; The chemical nature of catalase. Advance Enzymol. 1 163
    [Google Scholar]
  38. Yagi K., Matsuoka Y., Kuyama S., Tada M. 1956; Preparation of flavin adenine dinucleotide from Eremothecium ashbyi. J. Biochem., (Tokyo) 43 93
    [Google Scholar]
  39. Yaw K. E. 1952; Production of riboflavin by Eremothecium ashbyi grown in a synthetic medium. Mycologia 44 307
    [Google Scholar]
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