1887

Abstract

SUMMARY: Mutant staphylococci with impaired respiration, induced by ultraviolet radiation, were used for testing various inhibitors with already known mechanisms of action. Inhibitors of protein synthesis (the tetracyclins) affected the growth of parent and mutant cultures to the same degree. To the inhibitors of cell-wall synthesis (the penicillins) mutant staphylococci were more resistant than the parents; this was related to the altered amino acid composition of cell-wall material in the mutants. On the other hand, the mutants were much more vulnerable than parents to the action of inhibitors which affected the nucleic acids—namely, mitomycin C (x 50 times), actinomycin C (x 50). trypaflavine (x 300), nitrogen mustards (x 10-20). A specific thymine synthetase inhibitor (5-fluorodeoxyuridine) strongly inhibited the growth of parent staphylococci, but did not at all inhibit the growth of mutants. This points to some deficiency in the enzymic mechanism of synthesis of thymine in the DNA of mutants. Experiments with 5-bromodeoxyuridine suggest that the mutant staphylococci lacked the enzymic mechanism necessary for effective incorporation of halogenated pyrimidines into the DNA precursor pool.

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/content/journal/micro/10.1099/00221287-29-2-317
1962-10-01
2024-10-03
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References

  1. Cohen S., Flaks I., Babner H., Loeb M., Lichtenstein I. 1958; The mode of action of 5-fluorouracil and its derivatives. Proc. nat. Acad. Sci., Wash 44:1004
    [Google Scholar]
  2. Ephrussi B., Hottinguer H., Chimenes A. 1949; La mutation ‘petite colonie’. Ann. Inst. Pasteur 76:351
    [Google Scholar]
  3. Gale E. F. 1958; The mode of action of chloramphenicol. In the Ciba Foundation Symposium: Amino Adds and Peptides with Antimetabolic Activity p 19 London: Churchill;
    [Google Scholar]
  4. Gale E. F., Folkes J. P. 1953; Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. Biochem. J 53:493
    [Google Scholar]
  5. Gause G. F. 1960 The Search for New Antibiotics New Haven: Yale University Press;
    [Google Scholar]
  6. Gause G. F., Kochetkova G. V., Vladimirova G. B. 1957; Biochemical mutants of staphylococci with impaired respiration. Dokl. Acad. Sci. USSR 117:720
    [Google Scholar]
  7. Gause G. F., Kochetkova G. V., Vladimirova G. B. 1961; Biochemical changes accompanying impaired respiration in staphylococci. Dokl. Acad. Sci. USSR 139:223
    [Google Scholar]
  8. Horowitz J., Chargaff E. 1959; Massive incorporation of 5-fluorouracil into abacterial ribonucleic acid. Nature, Lond 184:1213
    [Google Scholar]
  9. Lindegren C. 1959; Cancer and the respiratory grana. Nature, Lond 184:397
    [Google Scholar]
  10. Lorkiewicz Z., Szybalski W. 1960; Genetic effects of halogenated thymidine analogs incorporated during thymidylate synthetase inhibition. Biochem. bSiophys. Res. Comm 2:413
    [Google Scholar]
  11. Morthland F. W., De Bruyn P. P., H. Smith N. 1954; Spectrophotometric studies on the interaction of nucleic acids with aminoacridines. Exp. Cell Res 7:201
    [Google Scholar]
  12. Reich E., Franklin R., Shatkin A., Tatum E. L. 1961; Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science 134:556
    [Google Scholar]
  13. Rogers H. I., Perkins H. R. 1959; Cell-wall mucopeptides of Staphylococcus aureus. Nature, Lond 184:520
    [Google Scholar]
  14. Shepherd C. I. 1958; Inhibitors of protein and nucleic acid synthesis in Aspergillus nidulans. J. gen. Microbiol 18:iv
    [Google Scholar]
  15. Shiba S., Terawaki A., Taguchi T., Kawamata I. 1959; Selective inhibition of formation of deoxyribonucleic acid in E. coli by mitomycin C. Nature, Lond 183:1056
    [Google Scholar]
  16. Strominger J. L., Park J. T., Thompson R. E. 1959; Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. J. biol. Chem 234:3263
    [Google Scholar]
  17. Szybalski W. 1960a; The mechanism of chemical mutagenesis with special reference to triethylene melamine action. Developments in Industrial Microbiology p 231 New York:
    [Google Scholar]
  18. Szybalski W. 1960b; Ultraviolet light sensitivity and other biological and physicochemical properties of halogenated DNA. Progress in Photobiology Copenhagen:
    [Google Scholar]
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