@article{mbs:/content/journal/micro/10.1099/00221287-29-2-311, author = "Woolley, D. and Jones, G. L. and Happold, F. C.", title = "Some Metabolic Differences Between Thiobacillus thioparus, T. denitrificans and T. thiocyanoxidans", journal= "Microbiology", year = "1962", volume = "29", number = "2", pages = "311-316", doi = "https://doi.org/10.1099/00221287-29-2-311", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-29-2-311", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Differences in behaviour of Thiobacillus thioparus, T. denitrificans and T. thiocyanoxidans were found in growth experiments carried out in thiosulphate or thiocyanate containing media and using aerobic or anaerobic conditions. These differences were: T. denitrificans and T. thioparus grew anaerobically in thiosulphate + nitrate and thiocyanate + nitrate media; T. thiocyanoxidans did not. During growth of T. thioparus in thiocyanate + nitrate medium, nitrite accumulated but thiocyanate was not completely oxidized; no nitrite accumulated during growth of T. denitrificans under similar conditions. After aerobic, serial subculture in thiosulphate medium T. denitrificans no longer grew anaerobically in thiosulphate + nitrate or thiocyanate + nitrate media whereas T. thioparus did. T. thioparus produced copious growth on thiosulphate agar slopes under aerobic conditions; T. denitrificans proved difficult to culture under such conditions, i.e. there was a failure to guarantee growth on each occasion, and where growth occurred this was poor. T. denitrificans did not produce the mucoid slime associated with T. thioparus cultures. T. thioparus accumulated and subsequently used up tetrathionate during aerobic growth in thiosulphate medium; T. denitrificans did not.", }