1887

Abstract

SUMMARY: Hydrolysates of the cell walls of 26 strains of group A streptococci were examined by paper chromatography. In all cases lysine, glutamic acid, alanine, glucosamine, muramic acid and rhamnose were found as the principal components. The mean relative proportion of lysine: glutamic acid:alanine was 1.1:1:4. Thus even when a number of strains of a particular bacterial species was tested, there was good uniformity in the cell-wall composition. Samples of cell-wall material which remained undigested after treatment with proteolytic enzymes all contained small amounts of several amino acids. It seems probable that a small amount of polypeptide or protein remained firmly attached to the cell walls examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-29-2-199
1962-10-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/29/2/mic-29-2-199.html?itemId=/content/journal/micro/10.1099/00221287-29-2-199&mimeType=html&fmt=ahah

References

  1. Armstrong J. J., Baddiley J., Buchanan J. G., Davison A. L., Kelemen M. V., Netjhaus F. C. 1959; Composition of teichoic acids from a number of bacterial walls. Nature, Lond 184:247
    [Google Scholar]
  2. Cummins C. S. 1956; The chemical composition of the bacterial cell wall Int. Rev. Cytol. 5:25
    [Google Scholar]
  3. Cummins C. S., Harris H. 1956; The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol 14:583
    [Google Scholar]
  4. Hayashi J. A., Barkulis S. S. 1959; Studies of streptococcal cell walls. III. The amino acids of trypsin-treated cell wall J. Bact 77:177
    [Google Scholar]
  5. Ikawa M. 1961; The partial chemical degradation of the cell walls of Lactobacillus plantarum, Streptococcus fa£calis, and Lactobacillus casei. J. biol. Chem 236:1087
    [Google Scholar]
  6. Kay R. E., Harris D. C., Entenman C. 1956; Quantification of the ninhydrin color reaction as applied to paper chromatography. Arch, biochem. biophys 63:14
    [Google Scholar]
  7. Lancefield R. C. 1954; Cellular constituents of group A streptococci concerned in antigenicity and virulence. In Streptococcal Infections p 3 Ed. by McCarty M. New York: Columbia University Press;
    [Google Scholar]
  8. Lancefield R. C., Dole V. P. 1946; The properties of T antigens extracted from group A streptococci. J. exp. Med 84:449
    [Google Scholar]
  9. McCarty M. 1952; The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus II. Nature of the cellular substrate attacked by the lytic enzymes J. exp. Med 96:569
    [Google Scholar]
  10. McCarty M., Lancefield R. C. 1955; Variations in the group specific carbohydrates of group A streptococci. I. Immunochemical studies on the carbohydrate of variant strains J. exp. Med 102:11
    [Google Scholar]
  11. Mickle H. 1948; Tissue disintegrator. J. R. micr. Soc 68:10
    [Google Scholar]
  12. Partridge S. M. 1949; Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars. Nature, Lond 164:443
    [Google Scholar]
  13. Roberts W. S. L., Stewart F. S. 1961; The sugar composition of streptococcal cell walls and its relation to haemagglutination pattern. J. gen. Microbiol 24:253
    [Google Scholar]
  14. Salton M. R. J. 1953; Studies of the bacterial cell wall. IV. The composition of the cell walls of some gram-positive and gram-negative bacteria Biochitn. biophys. Acta 10:512
    [Google Scholar]
  15. Salton M. R. J. 1956; Bacterial cell walls. Symp. Soc. gen. Microbiol 6:81
    [Google Scholar]
  16. Salton M. R. J. 1960; Surface layers of the bacterial cell. In The Bacteria Vol. I p.97 Edited by Gunsalus I. C., Stanier R. Y. New York and London: Academic Press;
    [Google Scholar]
  17. Schwab J. H., Cromartie W. J., Roberson B. S. 1959; Identification of a toxic cellular component of group A streptococci as a complex of specific C polysaccharide and a protein. J. exp. Med 109:43
    [Google Scholar]
  18. Wiley G. G., Wilson A. T. 1961; The occurrence of two M antigens in certain group A streptococci related to type 14. J. exp. Med 113:451
    [Google Scholar]
  19. Work E. 1957; Biochemistry of the bacterial cell wall. Nature, Lond 179:841
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-29-2-199
Loading
/content/journal/micro/10.1099/00221287-29-2-199
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error