1887

Abstract

SUMMARY: A streptomycin-resistant strain of , which requires haemin for aerobic growth, grew either aerobically or anaerobically in the absence of haemin provided the medium was supplemented with acetate or pyruvate; growth with these organic acids was increased by uracil and purines. The parent drug-sensitive strain grew aerobically without haemin but when grown anaerobically required either uracil or acetate or pyruvate. With both strains mevalonate replaced acetate and was about ten times more active.

The products of glucose fermentation by both strains showed no gross difference, lactate being predominant (about 85% of the glucose carbon); only small amounts of acetate were detected. Under aerobic conditions suspensions of the parent strain oxidized glucose to acetate which accumulated. The mutant strain oxidized glucose to acetate only when previously grown with haemin or when haemin was added to the suspension of organisms. When the organism was grown with acetate in place of haemin, lactate was the predominant product. The ability of the mutant to form sufficient acetate from glucose for biosynthetic purposes is apparently dependent on a functional electron transport chain involving haemoproteins. A nicotinamide-adenine dinucleotide-linked lactate dehydrogenase and a pyruvate oxidizing system are present in extracts of both organisms. The activity of these enzymes in the mutant strain was similar whether the organisms were grown on haemin or acetate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-29-1-157
1962-09-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/29/1/mic-29-1-157.html?itemId=/content/journal/micro/10.1099/00221287-29-1-157&mimeType=html&fmt=ahah

References

  1. Barritt M. M. 1936; The intensification of the Voges-Proskauer reaction by the addition of α-naphthol. J. Path. Bad. 42:441
    [Google Scholar]
  2. Barkkr S. B., Summerson W. H. 1941; The colorimetric determination of lactic acid in biological material. J. Mol. Chem. 138:535
    [Google Scholar]
  3. Jensen J., Thofern E. 1953; Chlorohamin (Ferri porphyrinchloride) als Bakterien-wuchsstoff. I. Z. Naturf. 8b:599
    [Google Scholar]
  4. Krebs H. A. 1937; Dismutation of pyruvic acid in gonococcus and staphylococcus. Biochem. J. 31:661
    [Google Scholar]
  5. Lascelles J. 1956; An assay of iron protoporphyrin based on the reduction of nitrate by a variant strain of Staphylococcus aureus; synthesis of iron protoporphyrin by suspensions of Rhodopseudomonas spheroides. J. gen. Microbiol. 15:404
    [Google Scholar]
  6. Lascelles J. 1960; The synthesis of enzymes concerned in bacteriochlorophyll formation in growing cultures of Rhodopseudomonas spheroides. J. gen. Microbiol. 23:487
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  8. Mickle H. 1948; Tissue disintegrator. J. Roy. Micr. Soc. 68 (Series III)10
    [Google Scholar]
  9. Popjak G., Cornforth J. W. 1960; The biosynthesis of cholesterol. Advanc. Enzymol. 22:281
    [Google Scholar]
  10. Reed L. J. 1957; The chemistry and function of lipoic acid. Advanc. Enzymol. 18:319
    [Google Scholar]
  11. Richardson G. M. 1936; The nutrition of Staphylococcus aureus. Necessity for uracil in anaerobic growth. Biochem. J. 30:2184
    [Google Scholar]
  12. Rose I. A. 1955 In Methods in Enzymology 1591 Ed. Colowick S. P., Kaplan N. O. New York: Academic Press Inc;
    [Google Scholar]
  13. Skeggs H. R., Wright L. D., Cresson E. L., Macrae G. D. E., Hoffman C. H., Wolf D. E., Folkers K. 1956; Discovery of a new acetate replacing factor. J. Bad. 72:519
    [Google Scholar]
  14. Wagner A. F., Folkers K. 1961; Discovery and chemistry of mevalonic acid. Advanc. Enzymol. 23:471
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-29-1-157
Loading
/content/journal/micro/10.1099/00221287-29-1-157
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error