SUMMARY: Conditions optimal for the laboratory culture of unpurified plasmodium were examined. The organism was grown in the laboratory for over 2 years, during which time regular sporulation occurred. Investigation of factors which affected sporulation suggested that this was induced by material present in older cultures; light did not precipitate it. Spore germination occurred readily in the laboratory; some experiments on germination were made. plasmodium was purified by a migration technique combined with antibiotic treatment. Two-member cultures were established with two yeasts and a sp. isolated from plasmodium, and with a baker's yeast. Several other organisms (including Gram-positive and Gram-negative bacteria and yeasts) were not satisfactory as associate organisms in two-member cultures. Satisfactory axenic culture was not obtained, slow growth for a few weeks only being obtained on an autoclaved suspension of baker's yeast.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error