The Influence of the Growth Medium on the Demonstration of a Group D Antigen in Faecal Streptococci Free

Abstract

SUMMARY: When grown under certain conditions some group D streptococci did not yield HCl extracts which would react serologically with group D antisera. By varying the composition of the medium, it was shown that the optimum yield of group D antigen was obtained when the organisms were grown in an unbuffered medium containing 5-10 g. glucose/l. where the ultimate pH of the culture was 4.0 to 4.2. In a buffered medium containing a low glucose concentration the final pH value of the culture remained above 6.0 and poor yields of group D antigen in the acid extract were obtained with , and

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-28-4-701
1962-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/28/4/mic-28-4-701.html?itemId=/content/journal/micro/10.1099/00221287-28-4-701&mimeType=html&fmt=ahah

References

  1. Bailey R. W., Oxford A. E. 1959; The nature of the capsular polysaccharide of the dextran-producing organisms Leuconostoc mesenteroides, L. dextranicum and Streptococcus bovis. J. gen. Microbiol. 20:258
    [Google Scholar]
  2. Barnes E. M., Ingram M. 1955; The identity and origin of faecal streptococci in canned hams. Ann. Inst. Pasteur, Lille 7:115
    [Google Scholar]
  3. Buttiaux R. 1955; Contribution to the discussion. Ann. Inst. Pasteur, Lille 7:101
    [Google Scholar]
  4. Deibel R. H., Evans J. B., Niven C. F. Jun. 1958; Lipoic acid requirement for anaerobic utilization of pyruvate as an energy source by Streptococcus faecalis. Bad. Proc.114
    [Google Scholar]
  5. Elliott S. D. 1960; Type and group polysaccharides of group D streptococci. J. exp. Med. 111:621
    [Google Scholar]
  6. Higginbottom C., Wheater D. W. F. 1954; The incidence of Streptococcus bovis in cattle. J. agric. Sci. 44:434
    [Google Scholar]
  7. Hobson P. N., MacPherson M. J. 1954; Some serological and chemical studies on materials extracted from an amylolytic streptococcus from the rumen of sheep. Biochem. J. 57:145
    [Google Scholar]
  8. Lancefield R. C. 1933; A serological differentiation of human and other groups of haemolytic streptococci. J. exp. Med. 57:571
    [Google Scholar]
  9. Lancefield R. C. 1937 Unpublished data. Cited by Sherman, J. M. (1938)
  10. MacPherson M. J. 1953; Isolation and identification of amylolytic streptococci from the rumen of sheep. J. Path. Bad. 66:95
    [Google Scholar]
  11. Medrek T. F., Barnes E. M. 1962a; The biochemical and serological properties of strains of Strep, bovis and related organisms isolated from sheep and cattle. J. appl. Bad.25 (in the Press)
    [Google Scholar]
  12. Medrek T. F., Barnes E. M. 1962b; The distribution of group D streptococci in sheep and cattle. J. appl. Bad. 25: (in the Press)
    [Google Scholar]
  13. Morelis P., Colobert L. 1958; Un milieu selectif permettant l’identification et le denombrement rapides de Streptococcus faecalis. Ann. Inst. Pasteur 95:568
    [Google Scholar]
  14. Sharpe M. E., Fewins B. G. 1960; Serological typing of strains of Streptococcus faecium and unclassified group D streptococci isolated from canned hams and pig intestines. J. gen. Microbiol. 23:621
    [Google Scholar]
  15. Shattock P. M. F. 1949; The streptococci of group D; the serological grouping of Streptococcus bovis and observations on serologically refractory strains. J. gen. Microbiol. 3:80
    [Google Scholar]
  16. Sherman J. M. 1938; The enterococci and related streptococci. J. Bad. 35:81
    [Google Scholar]
  17. Skadhauge K. 1950 Studies on the Enterococci with Special Reference to the Serological Properties. Copenhagen: Einar Munksgaards Forlag;
    [Google Scholar]
  18. Smith F. R., Niven C. F. Jun., Sherman J. M. 1938; The serological identification of Streptococcus zymogenes with the Lancefield group D. J. Bad. 35:425
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-28-4-701
Loading
/content/journal/micro/10.1099/00221287-28-4-701
Loading

Data & Media loading...

Most cited Most Cited RSS feed