Further Studies on the Antigens of Paramecium aurelia with the Aid of Fluorescent Antibodies Free

Abstract

SUMMARY: A technique for sectioning fixed preparations of suitable for subsequent treatment with fluorescent antisera is described. Following application of specific fluorescein and rhodamine conjugates of paramecium antisera to sectioned paramecia, fluorescence was observed both on the surface structures (pellicle and cilia) and in the cytoplasm, but not in the macronucleus. Fluorescence of the cytoplasm was prevented by pretreating the sections with non-homologous, non-fluorescent antiserum. It was concluded that the immobilizing antigens were substances covering only the pellicle and cilia, and that the internal cytoplasmic antigens were relatively invariant in paramecia of diverse immobilization antigen type. Study of transforming organisms showed that new antigen appeared first on the pellicle, and later on the cilia. All the cilia of a given cell bore the same antigen or mixtures of antigen.

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/content/journal/micro/10.1099/00221287-28-4-617
1962-09-01
2024-03-19
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References

  1. Beale G. H. 1948; The process of transformation of antigenic type in Paramecium aurelia. variety 4. Proc. nat. Acad. Sci., Wash. 34:418
    [Google Scholar]
  2. Beale G. H. 1954 The Genetics of Paramecium aurelia. Cambridge University Press;
    [Google Scholar]
  3. Beale G. H. 1957; The antigen system of Paramecium aurelia. Int. Rev. Cytol. 6:1
    [Google Scholar]
  4. Beale G. H. 1958; The role of the cjloplasm in antigen determination in Paramecium aurelia. Proc. roy. Soc. B, 148:308
    [Google Scholar]
  5. Beale G. H., Kacser H. 1957; Studies on the antigens of Paramecium aurelia with the aid of fluorescent antibodies. J. gen. Microbiol. 17:68
    [Google Scholar]
  6. Bishop J. 1961; Purification of an immobilization antigen of Paramecium aurelia, variety 1. Biochim. biophys. Acta 50:471
    [Google Scholar]
  7. Chadwick C. S., McEntegart M. G., Nairn R. C. 1958; Fluorescent protein tracers: a trial of new fluorochromes and the development of an alternative to fluorescein. Immunology 1:315
    [Google Scholar]
  8. Coons A. H., Kaplan M. H. 1950; Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. J. exp. Med. 91:1
    [Google Scholar]
  9. Marshall J. M., Eveland W. C., Smith C. W. 1958; Superiority of fluorescein isothiocyanate (Riggs) for fluorescent antibody technic with a modification of the application. Proc. Soc. exp. Biol., N. Y. 98:898
    [Google Scholar]
  10. Preer J. 1959; Studies on the immobilization antigens of Paramecium. III. Properties. J. Immunol. 83:385
    [Google Scholar]
  11. Preer J. R., Preer L. B. 1959; Gel diffusion studies on the antigens of isolated cellular components of Paramecium. J. Protozool. 6:88
    [Google Scholar]
  12. Sonneborn T. M. 1950; Methods in the general biology and genetics of Paramecium aurelia. J. exp. Zool. 113:87
    [Google Scholar]
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