SUMMARY: The uptake of radioactivity from C-streptomycin, in a form which cannot be displaced from the organisms by unlabelled streptomycin, was determined in several species of bacteria and one yeast growing under standard conditions. This uptake showed a good correlation with the sensitivity of the organism to streptomycin, and in streptomycin-resistant organisms was about 1 % of that in a highly sensitive organism, The quantity of streptomycin taken up when growth ceased represented an intracellular concentration 10- to 50-fold higher than that in the growth medium. Environmental factors, such as anaerobiosis or presence of carbon monoxide, which increased the resistance of to streptomycin also resulted in decreased rates of uptake of C-streptomycin. When grew in medium containing the streptomycin antagonist 2--heptyl-4-hydroxyquinoline -oxide, the rate of uptake of radioactivity was reduced to about 30 % of that in absence of the antagonist; the rate at which harvested organisms oxidized succinate and malate was reduced to a similar extent.


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