1887

Abstract

SUMMARY: The induction of penicillinase in resistant to benzyl penicillin has been investigated. Inducers tested included several new penicillins derived from 6-aminopenicillanic acid, benzyl penicillin amide and cephalosporin C. Fifteen strains of were examined, one in detail and the others using only two or three inducers. All strains possessed basal penicillinase and all were inducible, although maximum enzyme levels varied somewhat from one strain to another.

Hydrolysis of the penicillins by staphylococcal penicillinase was investigated by using supernatant fluid from a cephalosporin C-induced culture of 524 as the source of enzyme. Under these conditions all the penicillins except methicillin were inactivated, although at different rates. Hydrolysis of methicillin was only detectable at much higher enzyme concentrations. Hydrolysis of cephalosporin C was not observed.

The use of penicillins for the treatment of staphylococcal infections is discussed in the light of these results.

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1962-07-01
2021-08-06
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References

  1. Abraham E. P., Newton G. G. F. 1956; A comparison of the action of penicillinase on benzylpenicillin and cephalosporin N and the competitive inhibition of penicillinase by cephalosporin C. Biochem. J. 63:628
    [Google Scholar]
  2. Abraham E. P., Newton G. G. F. 1961; The structure of cephalosporin C. Biochem. J. 79:377
    [Google Scholar]
  3. Batchelor F. R., Doyle F. P., Nayler J. H. C., Rolinson G. N. 1959; Synthesis of penicillin: 6-Aminopenicillanic acid in penicillin fermentations. Nature, Lond. 183:257
    [Google Scholar]
  4. Bondi A. Jun., Dietz C. C. 1945; Penicillin resistant staphylococci. Proc. Soc. exp. Biol., N.Y. 60:55
    [Google Scholar]
  5. Bondi A., Dietz C. C. 1948; The susceptibility of penicillinase-producing bacteria to penicillin. 1. Factors influencing susceptibility. J. Bact. 55:843
    [Google Scholar]
  6. Bondi A., de Saint Phalle M., Kornblum J., Moat A. G. 1954; Factors influencing the synthesis of penicillinase by Micrococcus pyogenes. Arch. Biochem. 53:348
    [Google Scholar]
  7. Ford J. H. 1947; Hydroxylamine method of determining penicillins. Analyt. Chem. 19:1004
    [Google Scholar]
  8. Garrod L. P. 1960; Relative antibacterial activity of three penicillins. Brit. med. J. i:527
    [Google Scholar]
  9. Geronimus L. H. 1960; Inoculum size and apparent sensitivity of staphylococci to penicillins. New Engl. J. Med. 263:349
    [Google Scholar]
  10. Geronimus L. H., Cohen S. 1957; Induction of staphylococcal penicillinase. J. Bact. 73:28
    [Google Scholar]
  11. Geronimus L. H., Cohen S. 1958; Further evidence for inducibility of staphylococcal penicillinase. J. Bact. 76:117
    [Google Scholar]
  12. Henry R. J., Housewright R. D. 1947; Studies on penicillinase. II. Manometric method of assaying penicillinase and penicillin, kinetics of the penicillin-penicillinase reaction and the effects of inhibitors on penicillinase. J. biol. Chem. 167:559
    [Google Scholar]
  13. Holysz R. P., Stavely H. E. 1950; Carboxy derivatives of benzylpenicillin. J. Amer. chem. Soc. 72:4760
    [Google Scholar]
  14. Kantorowicz O. 1951; Shaking apparatus for the aeration of bacterial cultures. J. gen. Microbiol. 5:276
    [Google Scholar]
  15. Leitner F., Cohen S. 1960; Effect of pH on constitutive and inducible penicillinase synthesis in Staphylococcus aureus. Bact. Proc.150
    [Google Scholar]
  16. Manson E. E. D., Pollock M. R. 1953; The thermostability of penicillinase. J. gen. Microbiol. 8:163
    [Google Scholar]
  17. Novick R. P. 1962; Staphylococcal penicillinase and the new penicillins. Biochem. J. (in the Press)
    [Google Scholar]
  18. Perret C. J. 1954; Iodometric assay of penicillinase. Nature, Lond. 174:1012
    [Google Scholar]
  19. Pollock M. R. 1950; Penicillinase adaptation in B. cereus: Adaptive enzyme formation in the absence of free substrate. Brit. J. exp. Path. 31:739
    [Google Scholar]
  20. Pollock M. R. 1952; Penicillinase adaptation in Bacillus cereus: An analysis of the three phases in the response of logarithmically growing cultures to induction of penicillinase formation by penicillin. Brit. J. exp. Path. 33:587
    [Google Scholar]
  21. Pollock M. R. 1957; The activity and specificity of inducers of penicillinase production in Bacillus cereus, strain NRRL 569. Biochem. J. 66:419
    [Google Scholar]
  22. Pollock M. R. 1961; Exo-enzymes. The Bacteria Vol. 4 Gunsalus I. C., Stanier R. Y. New York: Academic Press;
    [Google Scholar]
  23. Pollock M. R., Torriani A-M. 1953; Purification et caractéristiques physicochimiques de la pénicillinase de Bacillus cereus. C.R. Acad. Sci., Paris 237:276
    [Google Scholar]
  24. Rogers H. J. 1945; The conditions controlling the production of hyaluronidase by micro-organisms grown in simplified media. Biochem. J. 39:435
    [Google Scholar]
  25. Rogers H. J. 1953; Variant populations within a hyaluronidase producing culture of Staphylococcus aureus. J. Path. Bact. 66:545
    [Google Scholar]
  26. Rogers H. J. 1954; The rate of formation of hyaluronidase, coagulase and total extracellular protein by strains of Staphylococcus aureus. J. gen. Microbiol. 10:209
    [Google Scholar]
  27. Rolinson G. N., Stevens S., Batchelor F. R., Wood J. C., Chain E. B. 1960; Bacteriological studies on a new penicillin—BRL 1241. Lancet ii:564
    [Google Scholar]
  28. Rowley D., Cooper P. D., Roberts P. W., Lester Smith E. 1950; The site of action of penicillin. 1. Uptake of penicillin on bacteria. Biochem. J. 46:157
    [Google Scholar]
  29. Steinman H. G. 1961a; Determination of the rate of enzymatic hydrolysis of 6-amino penicillanic acid. Fed. Proc. 20:232
    [Google Scholar]
  30. Steinman H. G. 1961b; Factors modifying induced formation of penicillinase in Staphylococcus aureus. J. Bact. 81:895
    [Google Scholar]
  31. Wildhack J., Ceci C. N., Moat A. G., Bondi A. 1960; Relationship between penicillinase inducibility and penicillinase resistance. Bact. Proc.129
    [Google Scholar]
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