1887

Abstract

SUMMARY: Suspensions of containing less than one viable bacterium per ten protozoa were prepared by aerobic incubation of the protozoa with penicillin, streptomycin, dihydrostreptomycin and neomycin in the presence of autoclaved rumen fluid and rice starch grains. Unfortunately these almost axenic Entodinia could be maintained alive for only 3-4 days. Replacement of the rice starch grains in the medium used for bacterial removal by soluble starch produced almost bacteria-free protozoa which were devoid of food storage materials and died more rapidly than usual. The life of these protozoa was prolonged by adding any of the following: rice starch grains, soluble starch, maltose, glucose and some other sugars.

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/content/journal/micro/10.1099/00221287-28-2-271
1962-06-01
2021-08-04
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References

  1. Abou Akkada A. R., Howard B. H. 1960; The biochemistry of rumen protozoa. 3. The carbohydrate metabolism of Entodinium. Biochem. J 76:445
    [Google Scholar]
  2. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci 36:205
    [Google Scholar]
  3. Bryant M. P., Robinson I. M. 1961; An improved non-selective culture medium for ruminal bacteria and its use in determining diurnal variation in numbers of bacteria in the rumen. J. Dairy Sci 44:1446
    [Google Scholar]
  4. Coleman G. S. 1958; Maintenance of oligotricli protozoa from the sheep rumen. in vitro. Nature, Lond 182:1104
    [Google Scholar]
  5. Coleman G. S. 1960a; The cultivation of sheep rumen oligotrich protozoa in vitro. J. gen. Microbiol 22:555
    [Google Scholar]
  6. Coleman G. S. 1960b; Effect of penicillin on the maintenance of rumen oligotrich protozoa. Nature, Lond 187:518
    [Google Scholar]
  7. Corliss J. O. 1959; An illustrated key to the higher groups of the ciliated protozoa, with definition of terms. J. Protozool 6:265
    [Google Scholar]
  8. King K. W., Smith P. H. 1955; Comparisons of two media proposed for the isolation of bacteria from the rumen. J. Bact 70:726
    [Google Scholar]
  9. Mrak E. M., Phaff H. J. 1948; Yeasts. Annu. Rev. Microbiol 2:1
    [Google Scholar]
  10. Oxford A. E. 1951; The conversion of certain soluble sugars to a glucosan by holotrich ciliates in the rumen of sheep. J. gen. Microbiol 5:83
    [Google Scholar]
  11. Oxford A. E. 1955; The bacteriology and protozoology of ruminant digestion. J. Sci. Food Agric 6:413
    [Google Scholar]
  12. Oxford A. E. 1958; Bloat in cattle. 9. Some observations on the culture of the cattle rumen ciliate Epidinium, ecaudatum (Crawley) occurring in quantity in cows fed on red clover (Trifolium pratense L.). N.Z. J. Agric. Res 1:809
    [Google Scholar]
  13. Stone H. W., Beeson C. 1936; Preparation and storage of standard chromous sulphate solution. Industr. Engng Chem. (Anal.) 8:188
    [Google Scholar]
  14. Sugden B. 1953; The cultivation and metabolism of oligotrich protozoa from the sheep’s rumen. J. gen. Microbiol 9:44
    [Google Scholar]
  15. Wegner G. H., Foster E. M. 1960; Fatty acid requirements of certain rumen bacteria. J. Dairy Sci 43:566
    [Google Scholar]
  16. Wilson M. K., Briggs C. A. E. 1955; The normal flora of the bovine rumen. 2. Quantitative bacteriological studies. J. appl. Bact 18:294
    [Google Scholar]
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