1887

Abstract

SUMMARY: The enzymic conversion of citrulline argininosuccinate arginine in wild-type has been shown to be essentially like that in mammalian tissues in all respects tested. The enzymes responsible for the two reactions (condensing enzyme and argininosuccinase) have been partially separated. Five mutants at the locus have normal arginino-succinase and little or no condensing activity. The lack of condensing activity appears to be due to a simple absence of enzyme, alternatives such as inhibitor production or increased ATPase competition having been ruled out. Small amounts of apparent condensing activity, detected in the substrate-disappearance assay, were shown to be due to side reactions. When grown at the usual high arginine concentrations, three mutants at the locus, which are known to lack argininosuccinase, have normal condensing activity. However, when strains are grown at low arginine concentrations, the resulting extracts have very little condensing activity. This also appears to be due to a simple loss of condensing enzyme. It has not been determined whether the low activity is a specific secondary effect of on the condensing enzyme, or whether it is merely a non-specific effect of the inadequate supplementation, which might cause reductions in many enzyme activities. Despite its very low condensing activity grown at low arginine concentrations must be active at some time , since its mycelium accumulates argininosuccinate but not citrulline. In contrast, an double mutant, grown at low arginine concentrations, must be inactive , since it accumulates citrulline but not argininosuccinate. It is concluded that is probably the primary locus controlling the synthesis of condensing enzyme.

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/content/journal/micro/10.1099/00221287-28-2-215
1962-06-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-28-2-215
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