%0 Journal Article %A Robson, Jun E. %A Stockley, Hazel M. %T Sulphydryl Metabolism of Fungi Grown in Submerged Culture %D 1962 %J Microbiology, %V 28 %N 1 %P 57-68 %@ 1465-2080 %R https://doi.org/10.1099/00221287-28-1-57 %I Microbiology Society, %X SUMMARY: Radioactive phenyl mercuric chloride (labelled with 203Hg) was used to measure-SH groups in the mycelium and in the medium. Tritiated phenyl mercuric chloride was used as a quantitative cytological reagent. The changes in -SH concentration of the fungus were not always directly related to the changes in total S in Aspergillus niger, Penicillium chrysogenum 49.133, Eremothecium ashbyii and Candida albicans. A peak in -SH concentration occurred in the cells of these organisms during the early growth phase. Extracellular -SH compounds were produced by all these organisms at some time during development. Cytological studies showed differences in the distribution of -SH compounds in the cell wall and cytoplasm. E. ashbyii and C. albicans had a strong -SH reaction in the cell wall, A. niger and P. chrysogenum 49.133 a weak one. Sulphydryl compounds were most concentrated in areas of hyphal bud formation in E. ashbyii but were not concentrated at the hyphal tip. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-28-1-57