SUMMARY: Radioactive phenyl mercuric chloride (labelled with Hg) was used to measure -SH groups in the mycelium and in the medium. Tritiated phenyl mercuric chloride was used as a quantitative cytological reagent. The changes in -SH concentration of the fungus were not always directly related to the changes in total S in and A peak in -SH concentration occurred in the cells of these organisms during the early growth phase. Extracellular -SH compounds were produced by all these organisms at some time during development. Cytological studies showed differences in the distribution of -SH compounds in the cell wall and cytoplasm. and had a strong -SH reaction in the cell wall, and a weak one. Sulphydryl compounds were most concentrated in areas of hyphal bud formation in but were not concentrated at the hyphal tip.


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