1887

Abstract

SUMMARY: A comparison has been made between the chemical composition of the cell walls of strains from the genera and , and the antigenic composition of the same cell-wall fractions as judged by an agglutination test. A common antigenic component was identified in all those strains of corynebacteria, mycobacteria and nocardias which have arabinose and galactose as their principal cell-wall sugars. Some strains of corynebacteria, and 3 strains of which have a different pattern of cell-wall components, appear to lack this cell-wall antigen. The antigen was not present in the cell walls of 8 strains of actinomyces and 6 of arthrobacter. Seven of the 8 strains of actinomyces had a cell-wall composition identical with that previously described for , and these 7 strains formed a homogeneous group serologically: the other actinomyces strain differed in cell-wall wall composition and its cell walls did not agglutinate with the ‘israelii’ serum, nor did those of any of the 6 strains of arthrobacter, although some of the latter resemble in cell-wall structure. These results show that the antigenic studies tended to confirm relationships suggested by cell-wall composition alone, especially among corynebacteria, nocardias and mycobacteria: they also suggest that as at present constituted is a mixture of species of diverse origin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-28-1-35
1962-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/28/1/mic-28-1-35.html?itemId=/content/journal/micro/10.1099/00221287-28-1-35&mimeType=html&fmt=ahah

References

  1. Barksdale W. L., Li Iv., Cummins C. S., Harris H. 1957; The mutation of Corynebacterium pyogenes to Corynebaderiun haemolyticum. J. gen. Microbiol. 16:749
    [Google Scholar]
  2. Bedson S. P., Baryvell C. F., King E. J., Bishop L. W. J. 1949; The laboratory diagnosis of lymphogranuloma veneieum. J. clin. Path. 2:241
    [Google Scholar]
  3. Cummins C. S. 1954; Some observations on the nature of the antigens in the cell wall of Corynebacterium diphtheriae. Brit. J, exp. Path. 35:166
    [Google Scholar]
  4. Cummins C. S., Harris H. 1956a; The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol. 14:583
    [Google Scholar]
  5. Cummins C. S., Harris H. 1956b; The relationship between certain members of the staphylococcus-micrococcus group as shown by their cell wall composition. Int. Bull. Bad. Nomend. 6:111
    [Google Scholar]
  6. Cummins C. S., Harris H. 1958; Studies on the cell wall composition and taxonomy of actinomycetales and related groups. J. gen. Microbiol. 18:173
    [Google Scholar]
  7. Cummins C. S., Harris II. 1959a; Cell wall composition in strains of Actinomyces isolated from human and bovine lesions. J. gen. Microbiol. 21:ii
    [Google Scholar]
  8. Cummins C. S., Harris H. 1959b; Taxonomic position of Arthrobacter. Nature, Lond. 184:831
    [Google Scholar]
  9. Heidelberger M., Menzel A. E. O. 1937; Specific and non-specific cell polysaccharides of a human strain of tubercle bacillus, 1137. J. biol. Chem. 118:79
    [Google Scholar]
  10. Hewitt L. F. 1947; Serological typing of C. diphtheriae. Brit. J. exp. Path. 28:338
    [Google Scholar]
  11. Karjala S. A., Heidelberger M. 1941; Specific and non-specific cell polysaccharides of an avian strain of tubercle bacillus. J. biol. Chem. 137:189
    [Google Scholar]
  12. Kirsh D., Shepard C. 1961; Se’ological relationships among mycobacteria. Fed. Proc. 20:16
    [Google Scholar]
  13. Kotani S., Kitaura T., Ilirano T., Tanaka A. 1959; Isolation and chemical composition of the cell walls of BCG. Biken’s J. 2:129
    [Google Scholar]
  14. Kotani S., Kitaura T., Higashigawa M., Kato K., Mori Y., Matsubara T., Tsujimoto T. 1960; Studies on the immunological properties of the cell wall, particle and soluble-fractions of sonicated BCG cells. Bikens J. 3:159
    [Google Scholar]
  15. II. Ludwig E., Hutchinson W. G. 1949; A serological study of selected species of actinomyces. J. Bad. 58:89
    [Google Scholar]
  16. Menzel A. E. O., Heidelberger M. 1939; Specificand non-specific cell polysaccharides of a bovine strain of tubercle bacillus. J. biol.Chem. 127:221
    [Google Scholar]
  17. Mickle H. 1948; A tissue disintegrator. J. roy. micr. Soc. 68:10
    [Google Scholar]
  18. Pine L., Howell A., Watson Sarah J. 1960; Studies of the morphological,physio logical and biochemical characters of Actinomyces bovis. J. gen. Microbiol. 23:403
    [Google Scholar]
  19. Schneidau J. D., Schaffer M. F. 1960; Studies on Nocardia and other Actinomycetales. II. Antigenic relationships shown by slide agglutination tests. Amer. J. resp. Dis. 82:64
    [Google Scholar]
  20. Shepard C. C., Kirsii D. 1961; Disruption of mycobacteria and their stainability by fluorescent antibody. Fed. Proc. 20:16
    [Google Scholar]
  21. Siebert F. B., Pederson K. O., Tiselius A. 1938; Molecular weight, electrochemical and biological properties of tuberci lin protein and polysaccharide molecules. J. exp. Med. 68:413
    [Google Scholar]
  22. Slavin D. 1950; Production of antisera in rabbits using calcium alginate as an antigen depot. Nature, Lond. 165:115
    [Google Scholar]
  23. Stacey M. L. 1955; Polysaccharides of the tubercle bacillus. Ciba Foundation Symposium on Experimental Tuberculosis. London: Churchill;
    [Google Scholar]
  24. Tennent D. M., Watson D. W. 1942; Molecular kinetic analyses and serological specificities of polysaccharides isola;ed from filtrates of the human tubercle bacillus and related species. J. Immunol. 45:179
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-28-1-35
Loading
/content/journal/micro/10.1099/00221287-28-1-35
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error