@article{mbs:/content/journal/micro/10.1099/00221287-27-3-521, author = "Yee, R. B. and Pan, Sylvia and Gezon, H. M.", title = "Effect of Chloramphenicol on Protein and Nucleic Acid Synthesis by Shigella flexneri", journal= "Microbiology", year = "1962", volume = "27", number = "3", pages = "521-527", doi = "https://doi.org/10.1099/00221287-27-3-521", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-27-3-521", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Resting organisms of Shigella flexneri 3 were able to synthesize protein and nucleic acids in a chemically defined medium. Protein synthesis was markedly inhibited while ribonucleic acid (RNA) synthesis was stimulated by low concentrations of chloramphenicol; deoxyribonucleic acid synthesis was inhibited slightly. Neither glycine, l-phenylalanine nor l-tyrosine annulled the action of the antibiotic. Inhibition of protein synthesis and stimulation of RNA synthesis in resting organisms of a chloramphenicol-resistant mutant of S. flexneri 3 was observed only in the presence of high concentrations of chloramphenicol. The results presented in this paper show that chloramphenicol suppresses the growth of S. flexneri 3 by interfering with the synthesis of protein. The stimulation of RNA synthesis suggests that chloramphenicol may exert its inhibitory action by promoting the formation of RNA with altered biological activity.", }