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SUMMARY: Two further groups of pigment mutants have been isolated from irradiated suspensions of the non-sulphur purple bacterium Rhodopseudomonas spheroides. Both contain the normal red carotenoid of the wild type, but in place of bacteriochlorophyll one group contains a derivative at the oxidation level of chlorophyll a, and the other a pigment at the oxidation level of protochlorophyll. Both these groups are non-photosynthetic. All attempts to produce stable photosynthetic mutants containing a pigment absorbing at the same wavelengths as chlorophyll a failed, although transient photosynthesis seemed to occur on several occasions. The relationship of these mutants to the biosynthetic pathway of bacteriochlorophyll suggested by Granick is discussed.
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