RT Journal Article SR Electronic(1) A1 Dawson, R. M. C. A1 White, R. W. A1 Freinkel, N.YR 1962 T1 The Fate of Mesoinositol during the Growth of an Inositol-dependent Yeast Kloeckera brevis JF Microbiology, VO 27 IS 2 SP 331 OP 339 DO https://doi.org/10.1099/00221287-27-2-331 PB Microbiology Society, SN 1465-2080, AB SUMMARY: An inositol-dependent yeast Kloeckera brevis was grown in a medium limited in inositol and the fate of the inositol determined. All of the inositol was taken in by the yeast and combined in an organic form. Fractionation of the yeast after disruption in a Hughes press showed that 31-38% of the combined inositol was soluble in trichloroacetic acid solution and 43-65% was soluble in neutral or acidified lipid solvents. The trichloroacetic acid extract contained three inositol components; one minor one was identified as inositol monophosphate. The major component was a neutral derivative of mesoinositol which was not identified as any known naturally-occurring form of combined inositol. The lipid extracted with neutral solvents contained an inositide whose properties were those of phosphatidyl inositol. The lipid inositol extracted with acidified solvents appeared to be present in a lipoprotein., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-27-2-331