%0 Journal Article %A Holt, L. B. %T The Culture of Bordetella pertussis %D 1962 %J Microbiology, %V 27 %N 2 %P 323-325 %@ 1465-2080 %R https://doi.org/10.1099/00221287-27-2-323 %I Microbiology Society, %X SUMMARY: Bordetella pertussis grew profusely and retained its normal minute coccobacillary form when grown under 0.4 mm. of fluid lying on a Cohen & Wheeler agar medium + charcoal. Microbes grown in this way were fully antigenic in terms of the mouse protection test. It was easy to obtain harvests of 1.2 x 1011 organisms/ml. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-27-2-323