SUMMARY: will grow in simple defined media containing proline or glutamate as sole carbon source; in these media the organisms grew at 0.7 and 0.9 doublings/hr., respectively. Since most of the proline fed to the organisms is converted endogenously to cell material via glutamate, an attempt was made to see what reaction limited growth when the organisms were growing in proline as sole carbon source. The results suggest that the step which controls the conversion of proline to glutamate endogenously is the rate-limiting step. The uptake of proline from the medium to the free amino-acid pool did not appear to be rate limiting under these conditions. There was no evidence that the need to synthesize additional enzyme proteins (the proline degradative pathway) was responsible for the decreased growth rate of organisms growing on proline alone.


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