1887

Abstract

SUMMARY: Hitherto only limited multiplication of has been obtained in cell cultures; in cultures of rat fibroblasts (strain 14 pf) used by Garbutt, Rees & Barr (1958) growth of bacilli was limited to one or two generations. The present work shows that more continuous intracellular growth of can be achieved in cultures of rat fibroblasts by repeatedly subculturing the infected cells. The results suggest that multiplication of the bacteria is maintained only when a high proportion (50-75%) of the infected cells are transferred at each subculture. In one experiment, continued for 156 days, the increase in the number of bacteria was equivalent to 8 generations and the bacteria recovered from the cells were still infectious for mice. Quantitative electron microscopy also was used to follow the viability of bacteria from the cell cultures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-27-2-259
1962-02-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/27/2/mic-27-2-259.html?itemId=/content/journal/micro/10.1099/00221287-27-2-259&mimeType=html&fmt=ahah

References

  1. Balfour-Jones S. E. B. 1937; The experimental transmission of rat leprosy to the golden hamster (Cricetus auratus) . J. Path. Bact 45:739
    [Google Scholar]
  2. Ehrmann R. L., Gey G. O. 1953; The use of cell colonies on glass for evaluating nutrition and growth in roller-tube cultures. J. nat. Canc. Inst 13:1099
    [Google Scholar]
  3. Garbutt E. W., Rees R. J. W., Barr Y. M. 1958; Multiplication of rat-leprosy bacilli in cultures of rat fibroblasts. Lancet ii:127
    [Google Scholar]
  4. Gey G. O., Hanks J. H., Barrett R. 1948; Retardation of growth and metabolism of normal and malignant cells during continuous cultivation. Growth 12:69
    [Google Scholar]
  5. Hanks J. H., Gray C. T. 1954; The application of metabolic studies to leprosy research. Int. J. Leprosy 22:147
    [Google Scholar]
  6. Hart P. D’Arcy, Rees R. J. W. 1960; Effect of macrocyclon in acute and chronic pulmonary tuberculous infection in mice as shown by viable and total bacterial counts. Brit. J. exp. Path 41:414
    [Google Scholar]
  7. Morgan J. F., Morton H. J., Parker R. C. 1950; Nutrition of animal cells in tissue culture. 1. Initial studies on a synthetic medium. Proe. soc. exp. Biol., N.Y 73:1
    [Google Scholar]
  8. Rees R. J. W. 1957a; The chemotherapeutic activity of Triton WR 1339 and macro-cyclon in murine leprosy. Amer. Rev. Tuberc 76:915
    [Google Scholar]
  9. Rees R. J. W. 1957b; Some experimental approaches to leprosy. E. Afr. med. J 34:361
    [Google Scholar]
  10. Rees R. J. W., Wong P. C. 1958; Limited multiplication of M. lepraemurium in tissue culture. Nature, Lond 181:359
    [Google Scholar]
  11. Rees R. J. W., Valentine R. C., Wong P. C. 1960; Application of quantitative electron microscopy to the study of Mycobacterium lepraemurium and M. leprae . J. gen. Microbiol 22:443
    [Google Scholar]
  12. Wallace J. H., Elek S. D., Hanks J. H. 1958; Limited multiplication of Mycobacterium lepraemurium in cell cultures. Proe. Soc. exp. Biol., N.Y 97:101
    [Google Scholar]
  13. Wong P. C. 1957 Study of the behaviour of Mycobacterium lepraemurium in the infected animal and in tissue culture Ph.D. Thesis University of London;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-27-2-259
Loading
/content/journal/micro/10.1099/00221287-27-2-259
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error