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SUMMARY: The effect of certain carbohydrates, nitrogen compounds and accessory growth factors on the growth and sporulation of several strains of Penicillium vermiculatum and P. wortmanni, as well as of many commonly occurring non-perithecial species, was studied. An initial low concentration of soluble sugar was conducive to perithecial formation in the two sexual species, and perithecial growth was best with polysaccharides as carbon source. Peptone was the most favourable nitrogen source; asparagine exerted an inhibitory effect in many cases. Of six B-group vitamins tested only thiamine had any marked effect. Similar effects of the nutritional factors were seen with the non-perithecial species generally.
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