1887

Abstract

SUMMARY: The production of polygalacturonase in associations of nodule bacteria and seedlings of leguminous plants was investigated. Plants and bacteria were combined in the following ways: ) different plant species were combined with infective strains (isolated from the same cross inoculation group) as well as non-infective ones (from foreign groups); ) host plant species with different susceptibilities were combined with the same bacterial strain; ) bacterial strains with different in-fectivities, as measured by the number of infection sites, were combined with the same host species; ) clover strains which had lost their infectivity, as well as transformed and again infective subcultures of these strains, were tested on clover plants. The results indicate that infection of the seedlings was strongly correlated with the production of polygalacturonase. The conclusion is drawn that polygalacturonase plays an important part in the infection process. This function is thought to be a weakening of the cell wall of the root hair which would facilitate the bacterial invasion. The possible role also of indolyl-3-acetic acid in the infection process is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-26-3-521
1961-11-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/26/3/mic-26-3-521.html?itemId=/content/journal/micro/10.1099/00221287-26-3-521&mimeType=html&fmt=ahah

References

  1. Dadd A. H., Jacobs S. E. 1958; The uptake of mercury by seeds treated with mercuric chloride solution, and its subsequent release. J. appl. Bad 21:97
    [Google Scholar]
  2. Dorn M. 1956; Über den Einfluss phytopathogener Pilze auf die Entwicklung und Infektionskraft von drei Rhizobium-Arten. Zbl. Bakt (II) 109:120
    [Google Scholar]
  3. Ekdahl I. 1953; Studies on the growth and the osmotic conditions of root hairs. Symb. Bot. Upsaliens 11:6
    [Google Scholar]
  4. Fährakus G. 1957; The infection of clover root hairs by nodule bacteria studied by a simple glass slide technique. J. gen. Microbiol 16:374
    [Google Scholar]
  5. Fáhraeus G., Ljunggren H. 1959; The possible significance of pectic enzymes in root hair infection by nodule bacteria. Physiol. Plant 12:145
    [Google Scholar]
  6. Galston A. W., Purves W. K. 1960; The mechanism of action of auxin. Annu. Rev. PI. Physiol 11:239
    [Google Scholar]
  7. Georgi C. E., Beguin A. E. 1939; Heteroauxin production by efficient and inefficient strains of rhizobia. Nature, Lond 143:25
    [Google Scholar]
  8. Glasziou K. T. 1957; The effect of 8-indolylacetic acid on the binding of pectin methylesterase to the cell walls of tobacco pith. Aust. J. biol. Sei 10:337
    [Google Scholar]
  9. Humphrey B. A., Vincent J. U. 1959; Extracellular polysaccharides of Rhizobium. J. gen. Microbiol 21:477
    [Google Scholar]
  10. Jacobs S. E., Dadd A. H. 1959; Antibacterial substances in seed coats and their role in the infection of sweet peas by Corynebacterium fascians (Tilford) Dowson. Ann. appl. Biol 47:666
    [Google Scholar]
  11. Kefford N. P., Brockwell J., Zwar J. A. 1960; The symbiotic synthesis of auxin by legumes and nodule bacteria and its role in nodule development. Aust. J. biol. Sei 13:456
    [Google Scholar]
  12. Link G. K. K. 1937; Role of heteroauxones in legume nodule formation, beneficial host effects of nodules, and soil fertility. Nature, Lond 140:507
    [Google Scholar]
  13. Ljunggren H. 1961; Transfer of virulence in Rhizobium trifolii. Nature, Lond 191:623
    [Google Scholar]
  14. Ljunggren H., Fáhraeus G. 1959; Effect of Rhizobium polysaccharide on the formation of polygalacturonase in lucerne and clover. Nature, Lond 184:1578
    [Google Scholar]
  15. Nutman P. S. 1946; Variation within strains of clover nodule bacteria in the size of nodule produced and in the ‘ effectiviity ’ of the symbiosis. J. Bact 51:411
    [Google Scholar]
  16. Nutman P. S. 1956; The influence of the legume in root-nodule symbiosis. Biol. Rev 31:109
    [Google Scholar]
  17. Nutman P. S. 1959; Some observations on root-hair infection by nodule bacteria. J. exp. Bot 10:250
    [Google Scholar]
  18. Thornton H. G. 1936; The action of sodium nitrate upon the infection of lucerne root-hairs by nodule bacteria. Proc. Roy. Soc B 119:474
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-26-3-521
Loading
/content/journal/micro/10.1099/00221287-26-3-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error