1887

Abstract

SUMMARY: A cytopathogenic bovine orphan (CBO) virus isolated from a bovine with lumpy skin disease appears unrelated to any of the three groups of viruses now associated with this condition. The virus multiplies efficiently in cultures of whole chick embryo tissue and may be titrated by plaque counting methods. Tissue culture fluids contain infective particles of at least three sizes, with sedimentation constants of 460, 72 and 20 S. The intermediate particle, if spherical and lipid free, would be comparable in size to the virus of foot and mouth disease. Heterogeneity of the virus has also been demonstrated by plaque morphology, electrophoresis and chromatography on DEAE cellulose. It is adsorbed to fresh but not to receptor-destroying enzyme (RDE) treated red cells of chicken and goose, but haemagglutination has not been observed with cells of any of the species tested. The virus lacks enzyme activity comparable to that of influenza virus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-26-3-405
1961-11-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/26/3/mic-26-3-405.html?itemId=/content/journal/micro/10.1099/00221287-26-3-405&mimeType=html&fmt=ahah

References

  1. Alexander R. A., Plowright W., Haig D. A. 1957; Cytopathogenic agents associated with lumpy skin disease of cattle. Bull. epiz. Dis. Afr 5:489
    [Google Scholar]
  2. Bradish C. J., Brooksby J. B. 1960; Complement-fixation studies of the specificity of the interactions between components of the virus system of foot-and-mouth disease and its antibodies. J. gen. Microbiol 22:405
    [Google Scholar]
  3. Cooper P. D. 1955; A method for producing plaques in agar suspensions of animal cells. Virology 1:397
    [Google Scholar]
  4. Dulbecco R. 1952; The production of plaques in monolayer tissue cultures by single particles of an animal virus. Proc. nat. Acad. Sci. Wash 38:8747
    [Google Scholar]
  5. Peterson E. A., Sober H. A. 1956; Chromatography of protein. I. Cellulose ion exchange adsorbents J. Amer. chem. Soc 78:751
    [Google Scholar]
  6. Polson A., Cramer R. 1958; Zone electrophoresis of type I poliomyelitis virus. Biochim. biophys. Acta 29:187
    [Google Scholar]
  7. Polson A., Turner G. S. 1954; pH stability and purification of lumpy skin disease virus. J. gen. Microbiol 11:228
    [Google Scholar]
  8. Turner G. S., Kipps A., Polson A., van den Ende M. 1961; Neutralization of a cytopathogenic bovine orphan virus in tissue culture by heat stable Francis type inhibitors in normal animal sera. J. gen. Microbiol 26:415
    [Google Scholar]
  9. van den Ende M., Alexander R. A., Don P. A., Kipps A. 1948; Isolation in chick embryos of a filtrable agent possibly related etiologically to lumpy skin disease of cattle. Nature, Land 161:526
    [Google Scholar]
  10. van den Ende M., Don P. A., Kipps A. 1949; The isolation in eggs of a new filtrable agent which may be the cause of bovine lumpy skin disease. J. gen. Microbiol 3:174
    [Google Scholar]
  11. van den Ende M., Turner G. S. 1950; Further observations on a filtrable agent isolated from bovine lumpy skin disease. J. gen. Microbiol 4:225
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-26-3-405
Loading
/content/journal/micro/10.1099/00221287-26-3-405
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error