1887

Abstract

SUMMARY: Magnesium or certain other divalent metal ions are needed for the adsorption of phage anti-R to its host, a ‘rough’ strain of In medium containing magnesium ions the infected organisms burst after a latent period of about 20 min. at 37° but when infected organisms are diluted into medium without added divalent metal ions phage growth is considerably inhibited. A rapid increase in phage titre occurs when magnesium ions are added late, i.e. at some time after the end of the normal latent period, to dilute cultures of phage-infected organisms. During the interval between infection and this late addition of magnesium ions no appreciable numbers of infective intracellular phage were detected after ultrasonic disruption of the organisms. It is concluded that in addition to being an adsorption cofactor, magnesium functions at some late stage in phage development. Phage anti-R is probably related to ϕX 174; it consists of particles approximately 30 mμ in diameter and the intact phage reacts similarly with formaldehyde.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-26-2-313
1961-10-01
2021-07-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/26/2/mic-26-2-313.html?itemId=/content/journal/micro/10.1099/00221287-26-2-313&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1949; The stability of bacterial viruses in solutions of salts.. J. gen. Physiol. 32:579
    [Google Scholar]
  2. Adams M. H. 1950; Methods of study of bacterial viruses.. Meth. Med. Res. 11:1
    [Google Scholar]
  3. Albert A. 1950; Quantitative studies of the activity of naturally occurring substances for trace metals.. I. Amino acids having only two ionizing groups Biochem. J. 47:531
    [Google Scholar]
  4. Anderson T. F., Doermann A. H. 1952; The intracellular growth of bacteriophages.. II. The growth of T3 studied by sonic disintegration and by T 6-cyanide lysis of infected cells J. gen. Physiol. 35:657
    [Google Scholar]
  5. Elford W. J., Andrews C. H. 1932; The sizes of different bacteriophages.. Brit. J. exp. Path. 13:446
    [Google Scholar]
  6. Ellis E. L., Delbrück M. 1939; The growth of bacteriophage.. J. gen. Physiol. 22:365
    [Google Scholar]
  7. Fildes P. 1954; The relation of divalent metals to lysis of typhoid bacilli by bacteriophages.. Brit. J. exp. Path. 35:122
    [Google Scholar]
  8. Fildes P., Kay D., Joklik W. K. 1952; Divalent metals in phage production.. Symp. Soc. gen. Microbiol. 2:194
    [Google Scholar]
  9. Garen A. 1954; Thermodynamic and kinetic studies on the attachment of T1 bacteriophage to bacteria.. Biochem. biophys. Acta, 14:163
    [Google Scholar]
  10. Gratia A. 1940; Action des électrolytes sur les bactériophages et sur leur fixation par les bactéries sensibles.. Bull. Acad. Med. Belg. 5:232
    [Google Scholar]
  11. Kay D. 1952; The intracellular multiplication of coli bacteriophage T5st.. Brit. J. exp. Path. 33:236
    [Google Scholar]
  12. Kay D., Fildes P. 1950; The calcium requirement of a typhoid bacteriophage.. Brit. J. exp. Path. 31:338
    [Google Scholar]
  13. Luria S. E., Steiner D. L. 1954; The role of calcium in the penetration of bacteriophage T5 into its host.. J. Bact. 67:635
    [Google Scholar]
  14. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb. 38:732
    [Google Scholar]
  15. Mitchell P. 1949; A new technique for stirred aerated culture.. Nature, Lond. 164:846
    [Google Scholar]
  16. Monod J., Cohen-Bazire G., Cohn M. 1951; Sur la biosynthèse de la β-galactosidase (lactase) chez Escherichia coli. La speeificité de l’induction Biochem. biophys. Acta 7:585
    [Google Scholar]
  17. Puck T. T., Garen A., Cline J. 1951; The mechanism of virus attachment to host cells.. I. The role of ions in the primary reaction J. exp. Med. 93:65
    [Google Scholar]
  18. Rountree P. M. 1955; The role of divalent cations in the multiplication of staphylococcal bacteriophages.. J. gen. Microbiol. 12:275
    [Google Scholar]
  19. Sinsheimer R. L. 1959; Purification and properties of bacteriophage ɸX 174.. J. mol. Biol. 1:37
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-26-2-313
Loading
/content/journal/micro/10.1099/00221287-26-2-313
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error