1887

Abstract

SUMMARY: The formation of penicillinase, induced in a growing culture of 6346 by a single addition of benzylpenicillin, spontaneously ceases after about 3 hr. The enzyme continues, however, to be released into the medium, thus permitting the process of its liberation to be studied independently from its production. By using the escape into the medium of a normally intracellular, maltose-inducible α-glucosidase as a more sensitive indicator of cell damage than direct measurement of lysis, it was concluded that at least 40 % of the penicillinase is liberated from the cells without gross disorganization of their structure.

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/content/journal/micro/10.1099/00221287-26-2-239
1961-10-01
2024-04-25
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References

  1. Aizawa K. 1939; Studien über Carbohydrase.. I. Die fermentative Hydrolyse der p-nitrophenolglukoside J. Biochem. (Tokyo) 30:89
    [Google Scholar]
  2. Henry R. J., Housewright R. D. 1947; Studies on penicillinase.. II. Manometric method of assaying penicillinase and penicillin, kinetics of the penicillin-penicillinase reaction and the effects of inhibitors on penicillinase J. biol. Chern. 167:559
    [Google Scholar]
  3. Kushner D. J. 1960; An immunological study of Bacillus subtilis penicillinases.. J. gen. Microbiol. 23:381
    [Google Scholar]
  4. Kushner D. J., Pollock M. R. 1961; The liberation of penicillinase from Bacillus subtilis. 2. The location of cell-bound penicillinase J. gen. Microbiol. 26:255
    [Google Scholar]
  5. Lederberg J. 1950; The β-d-galactosidase of Escherichia coli, strain K-121 . J. Bact. 60:381
    [Google Scholar]
  6. Lowry O. H., Rosebrough M. J., Farr A. L., Randall R. S. 1951; Protein measurement with folin phenol reagent.. J. biol. Chem. 193:265
    [Google Scholar]
  7. Nomura M., Hosoda J. 1956; Nature of the primary action of the autolysin of B. subtilis. J. Bact 72:573
    [Google Scholar]
  8. Nomura M., Hosoda J., Yoshikawa H. 1958; Studies on amylase formation by Bacillus subtilis. The mechanism of amylase excretion and cellular structure of Bacillus subtilis J. Biochem. (Tokyo) 45:737
    [Google Scholar]
  9. Perret C. J. 1954; Iodometric assay of penicillinase.. Nature, Lond. 174:1012
    [Google Scholar]
  10. Pollock M. R. 1953; Stages in Enzyme Adaptation.. Symp. Soc. gen. Microbiol 3:150
    [Google Scholar]
  11. Pollock M. R. 1956; The cell-bound penicillinase of Bacillus cereus. J. gen. Microbiol. 15:154
    [Google Scholar]
  12. Pollock M. R. 1961a; The mechanism of liberation of penicillinase from Bacillus subtilis. J. gen. Microbiol. 26:267
    [Google Scholar]
  13. Pollock M. R. 1961b In The Bacteria 4 Chap. 12. Exo-enzymes. (I.C. GunsalusR. Y. Stanier, Eds.) New York: Academic Press. (In the Press.);
    [Google Scholar]
  14. Pollock M. R., Kramer M. 1958; Intermediates in the biosynthesis of bacterial penicillinase.. Biochem. J. 70:665
    [Google Scholar]
  15. Pollock M. R., Perret C. J. 1951; The relation between fixation of penicillin sulphur and penicillinase adaptation in B cereus. Brit. J. exp. Path. 32:387
    [Google Scholar]
  16. Pollock M. R., Torriani A. M. 1953; Purification et caractéristiques physiochimiques de la pénicillinase de Bacillus cereus. C.R. Acad. Sci., Paris, 237:276
    [Google Scholar]
  17. Rolinson C. N., Batchelor F. R., Butterworth D., Cameron Wood J., Cole M., Eustace G. C., Hart M. V., Richards M., Chain E. B. 1960; Formation of 6-aminopenicillanic acid from penicillin by enzymatic hydrolysis.. Nature, Lond. 187:236
    [Google Scholar]
  18. Weibull C. 1956; The nature of the ‘ ghosts ’ obtained by lysozyme lysis of B. megaterium. Exp. Cell Res. 10:214
    [Google Scholar]
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