1887

Abstract

SUMMARY: Serum-free chemically defined media were developed for cultivating several Leptospira strains. , Ruebeusch strain, was the main test strain. Esterified fatty acids (monoolein, monostearin, methyl palmitate, methyl oleate) replaced serum. Fe over a narrow range replaced haemin. Thiamine was essential. Other vitamins added to thiamine-containing medium (nicotinic acid, pantothenate, putrescine, -hydroxybenzoic acid, vitamin B) speeded or increased final growth. Acetate stimulated growth moderately. Ammonium sulphate was favourable over a wide concentration range.

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1961-10-01
2022-07-03
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