SUMMARY: Methods for the assay of sporidesmin and the sporidesmolides were worked out and used in the study of the production of these metabolites by (Commonwealth Mycological Institute, Kew, England, Herbarium no. 74473) growing on an enriched potato carrot medium. High yields of sporidesmin and the sporidesmolides were associated with high utilization of medium constituents. No increase in the quantity of sporidesmin isolated from unit volume of medium was observed after the third day of incubation, despite further rapid growth of the fungus; by contrast good correlation was observed between the weight of sporidesmolides produced and fungal growth. These results were successfully applied to the production of experimental quantities of these materials.


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