1887

Abstract

SUMMARY: A wild-type strain of (W) did not utilize glutamate or oxoglu-tarate as sole source of carbon for growth, but mutants able to grow on each of these compounds were isolated. The abilities to utilize glutamate and oxoglutarate did not necessarily accompany each other. The evidence presented supports the view-that both kinds of mutant involve changes in a permeation mechanism. The mutation to growth on glutamate was always accompanied by appearance of sensitivity to inhibition by 2-methyl--glutamic acid and by partial or even complete loss of glutamic acid decarboxylase. It is proposed that the permeation mechanism for glutamate also allows entry of 2-methylglutamate, a compound which prevents glutamine formation. The loss of glutamate decarboxylase remains unexplained.

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/content/journal/micro/10.1099/00221287-26-2-175
1961-10-01
2024-12-10
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References

  1. Ayengar P., Roberts E. 1952; Inhibition of utilization of glutamic acid by Lactobacillus arabinosus. Proc. Soc. exp. Biol., N.Y 79:476
    [Google Scholar]
  2. Davis B. D. 1948; The isolation of biochemically deficient mutants of bacteria by means of penicillin.. Proc. nat. Acad. Sci., Wash 35:1
    [Google Scholar]
  3. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12 . J. Bad 60:17
    [Google Scholar]
  4. Fry B. A. 1955; Glutamine synthesis by Micrococcus pyogenes var.. aureus. J. gen. Microbiol 59:579
    [Google Scholar]
  5. Giri K. V., Radhakrishnan A. N., Vaidyanathan C. S. 1952; A simple paper chromatographic method for the study of transamination reactions.. Nature, Lond 170:1025
    [Google Scholar]
  6. Halpern Y. S., Grossowicz N. 1956; Determination of l-glutamic acid by the use of l-glutamic acid decarboxylase from Mycobacterium phlei. Proc. Soc. exp. Biol., N.Y 91:370
    [Google Scholar]
  7. Halpern Y. S., Umbarger H. E. 1959; Glutamic decarboxylase—the limiting factor in the utilization of glutamate as a carbon source by Escherichia coli. Bact. Proc p 122
    [Google Scholar]
  8. Halpern Y. S., Umbarger H. E. 1960; Conversion of ammonia to amino groups in Escherichia coli. J. Bact 80:285
    [Google Scholar]
  9. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas.. Biochem. J 55:800
    [Google Scholar]
  10. Mehl J. W. 1945; The biuret reaction of proteins in the presence of ethylene glycol.. J. biol. Chem 157:173
    [Google Scholar]
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