SUMMARY Biochemical mutants of were obtained after treatment with ultraviolet irradiation or ethyleneimine. A specificity of the mutational process in a series of strains was observed, revealing itself mainly in formation of arginine-requiring mutants. Prototroph formation was shown to result from crosses of biochemical mutants with either the same or different amino acid requirements. Colonial morphology and antibacterial activity of some prototrophs were studied.


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