1887

Abstract

SUMMARY Two distinct temperate phages (594a, 594n) lysogenizing phage type 80/81 staphylococci and producing gains in sensitivity to typing phages 52 and 52A were found in lysogenic clones of a strain of staphylococcus of phage type 52/52A/80/81. When either of these phages infected type 80/81 cocci a new phage (594b) appeared in the lysates. This phage was unable to replicate in type 80/81 cocci but could grow in them when they had been lysogenized with phage 594a or 594n. The source of phage 594b was found to be recombination of phage 594a or 594n with a prophage present in cocci of phage type 80/81. This prophage was completely defective and could be demonstrated only by recombination. The gains in phage sensitivity that follow lysogenization of type 80/81 strains with the so-called ‘converting’ phages can be most satisfactorily explained on the basis of prophage substitution.

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/content/journal/micro/10.1099/00221287-26-1-111
1961-09-01
2024-04-24
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References

  1. Asheshov E. H., Rippon J. E. 1959; Changes in typing pattern of phage-type 80 staphylococci. J. gen. Microbiol 20:634
    [Google Scholar]
  2. Bertani G. 1958; Lysogeny. Advanc. Virus Res 5:151
    [Google Scholar]
  3. Bynoe E. T., Elder R. H., Comtois R. D. 1956; Phage-typing and antibiotic resistance of staphylococci isolated in a general hospital. Canad. J. Microbiol 2:346
    [Google Scholar]
  4. Cohen D. 1959; A variant of phage P2 originating in Escherichia coli strain B. Virology 7:112
    [Google Scholar]
  5. Gorrill R. H. 1957; Studies on lysogeny in staphylococci. J. gen. Microbiol 17:254
    [Google Scholar]
  6. Gorrill R. H., Gray R. A. 1956; The induction of bacteriophage in staphylococci. J. gen. Microbiol 14:167
    [Google Scholar]
  7. Hershey A. D. 1958; The production of recombinants in phage crosses. Cold Spr. Harb. Symp. quant. Biol 23:19
    [Google Scholar]
  8. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol 36:39
    [Google Scholar]
  9. Jacob F., Wollman E. L. 1959; The relationship between the prophage and the bacterial chromosome in lysogenic bacteria. Recent Progress in Microbiology Stockholm: Almquist and Wiksell;
    [Google Scholar]
  10. Rountree P. M. 1959; Changes in the phage-typing patterns of staphylococci following lysogenization. J. gen. Microbiol 20:620
    [Google Scholar]
  11. Swanstrom M., Adams M. H. 1951; Agar layer method for production of high titre phage stocks. Proc. Soc. exp. Biol., N.Y 78:372
    [Google Scholar]
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