RT Journal Article SR Electronic(1) A1 Bachrach, U. A1 Cohen, I.YR 1961 T1 Spermidine in the Bacterial Cell JF Microbiology, VO 26 IS 1 SP 1 OP 9 DO https://doi.org/10.1099/00221287-26-1-1 PB Microbiology Society, SN 1465-2080, AB SUMMARY The concentration of spermidine in extracts of various micro-organisms was determined; Neurospora crassa contained the highest concentrations of spermidine, while in Pseudomonas aeruginosa, Escherichia coli and Saccharomyces cerevisiae the concentration of spermidine was lower. Growing organisms and cell-free extracts of the micro-organisms tested were able to synthesize [14C]spermidine from dl-[2-14C]methionine. Cell-free extracts of Pseudomonas aeruginosa were most active in this respect. [14C]Spermidine formed by growing Bacillus subtilis was localized in both the protoplasts and the bacterial cell walls. The kinetics of the adsorption of [14C]spermidine on to cell walls and protoplasts of B. subtilis were also studied. The interpretation of these observations and their relation to the antibacterial effect and growth-promoting activity of spermidine are discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-26-1-1