@article{mbs:/content/journal/micro/10.1099/00221287-26-1-1, author = "Bachrach, U. and Cohen, I.", title = "Spermidine in the Bacterial Cell", journal= "Microbiology", year = "1961", volume = "26", number = "1", pages = "1-9", doi = "https://doi.org/10.1099/00221287-26-1-1", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-26-1-1", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY The concentration of spermidine in extracts of various micro-organisms was determined; Neurospora crassa contained the highest concentrations of spermidine, while in Pseudomonas aeruginosa, Escherichia coli and Saccharomyces cerevisiae the concentration of spermidine was lower. Growing organisms and cell-free extracts of the micro-organisms tested were able to synthesize [14C]spermidine from dl-[2-14C]methionine. Cell-free extracts of Pseudomonas aeruginosa were most active in this respect. [14C]Spermidine formed by growing Bacillus subtilis was localized in both the protoplasts and the bacterial cell walls. The kinetics of the adsorption of [14C]spermidine on to cell walls and protoplasts of B. subtilis were also studied. The interpretation of these observations and their relation to the antibacterial effect and growth-promoting activity of spermidine are discussed.", }