SUMMARY The concentration of spermidine in extracts of various micro-organisms was determined; contained the highest concentrations of spermidine, while in and the concentration of spermidine was lower. Growing organisms and cell-free extracts of the micro-organisms tested were able to synthesize [C]spermidine from -[2-C]methionine. Cell-free extracts of were most active in this respect. [C]Spermidine formed by growing was localized in both the protoplasts and the bacterial cell walls. The kinetics of the adsorption of [C]spermidine on to cell walls and protoplasts of were also studied. The interpretation of these observations and their relation to the antibacterial effect and growth-promoting activity of spermidine are discussed.


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