1887

Abstract

SUMMARY The concentration of spermidine in extracts of various micro-organisms was determined; contained the highest concentrations of spermidine, while in and the concentration of spermidine was lower. Growing organisms and cell-free extracts of the micro-organisms tested were able to synthesize [C]spermidine from -[2-C]methionine. Cell-free extracts of were most active in this respect. [C]Spermidine formed by growing was localized in both the protoplasts and the bacterial cell walls. The kinetics of the adsorption of [C]spermidine on to cell walls and protoplasts of were also studied. The interpretation of these observations and their relation to the antibacterial effect and growth-promoting activity of spermidine are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-26-1-1
1961-09-01
2022-10-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/26/1/mic-26-1-1.html?itemId=/content/journal/micro/10.1099/00221287-26-1-1&mimeType=html&fmt=ahah

References

  1. Ames B. N., Dubin D. T., Rosenthal S. M. 1958; Presence of polyamines in certain bacterial viruses. Science 127:814
    [Google Scholar]
  2. Ames B. N., Dubin D. T. 1960; The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J. biol. Chem 235:769
    [Google Scholar]
  3. Anand N., Davis B. D. 1960; Effect of streptomycin on Escherichia coli. Nature, Lond 185:22
    [Google Scholar]
  4. Anand N., Davis B. D., Armitage A. K. 1960; Uptake of streptomycin by Escherichia coli. Nature, Lond 185:23
    [Google Scholar]
  5. Anderson T. F. 1960; Bacterial viruses—structure and function. The Bacteria ed Gunsalus I. C., Stanier R. Y. 1409 New York: Academic Press Inc;
    [Google Scholar]
  6. Bachrach U. 1957; The degradation of glycine by Pseudomonas aeruginosa. Biochem. J 66:559
    [Google Scholar]
  7. Bachrach U. 1960; Metabolism of amines. 3. The degradation and synthesis of γ-aminobutyric acid by Pseudomonas aeruginosa. Biochem. J 77:417
    [Google Scholar]
  8. Bachrach U., Persky S., Razin S. 1960; Metabolism of amines. 2. The oxidation of natural poly amines by Mycobacterium smegmatis. Biochem. J 76:306
    [Google Scholar]
  9. Baker W., Harborne J. B., Ollis W. D. 1952; Characterisation of primary aliphatic’ amines by reaction with o-acetoacetylphenol and by paper chromatography. J. chem. Soc p 3215
    [Google Scholar]
  10. Bawden F. C., Pirie N. W. 1959; The infectivity and inactivation of nucleic acid preparations from tobacco mosaic virus. J. gen. Microbiol 21:438
    [Google Scholar]
  11. Cohen S. S. 1946; Streptomycin and desoxyribonuclease in the study of variations in the properties of a bacterial virus. J. biol. Chem 166:393
    [Google Scholar]
  12. Cohen S. S., Lichtenstein J. 1960; Polyamines and ribosome structure. J. biol. Chem 235:2112
    [Google Scholar]
  13. Davis B. D. 1949; The isolation of biochemically deficient mutants of bacteria by means of penicillin. Proc. nat. Acad. Sei., Wash 35:1
    [Google Scholar]
  14. Dubin D. T., Rosenthal S. M. 1960a; The acetylation of polyamines in Escherichia coli. J. biol. Chem 235:776
    [Google Scholar]
  15. Dubin D. T., Rosenthal S. M. 1960b; Polyamine glutathione conjugates in E. coli. Fed. Proc 19:1
    [Google Scholar]
  16. Dudley H. W., Rosenheim M. C., Rosenheim O. 1924; The chemical constitution of spermine. I. The isolation of spermine from animal tissues and the preparation of its salts Biochem. J 18:1263
    [Google Scholar]
  17. Dudley H. W., Rosenheim O. 1925; Notes on spermine. Biochem. J 19:1034
    [Google Scholar]
  18. Dudley H. W., Rosenheim O., Starling W. W. 1927; The constitution and synthesis of spermidine, a newly discovered base from animal tissues. Biochem. J 21:97
    [Google Scholar]
  19. Felsenfeld G., Huang S. 1960; The interaction of polynucleotides with metal ions, amino acids and polyamines. Biochem. biophys. Acta 37:425
    [Google Scholar]
  20. Fischer F. G., Bohn H. 1957a; Die Giftsekrete der Yogelspinnen. Liebigs Ann 603:232
    [Google Scholar]
  21. Fischer F. G., Bohn H. 1957b; Über die Bestimmung von Spermin, Spermidin und anderen biogenen Aminen nach papierelektrophoretischer Abtrennung und ihre Mengenverhältnisse in tierischen Organen. Hoppe-Seyl Z 308:108
    [Google Scholar]
  22. Fitz-James P. C. 1958; Studies on the morphology and nucleic acid content of proto-plasts of Bacillus megaterium. J. Bad 75:369
    [Google Scholar]
  23. Fraser D., Mahler H. R. 1958; Effect of diamines on the protoplast infecting agent derived from T2 bacteriophage. J. Amer. chem. Soc 80:6456
    [Google Scholar]
  24. Giri K. V., Radhakrishnan A. N., Vaidyanathan C. S. 1952; A simpler paper chromatographic method for the study of transamination reactions. Nature, Lond 170:1025
    [Google Scholar]
  25. Greene R. C. 1957; Incorporation of the carbon chain of methionine into spermidine. J. Amer. chem. Soc 79:3929
    [Google Scholar]
  26. Gurevitch J., Rozansky R., Weber D., Brzezinsky A., Eckerling B. 1951; The role of spermine in the inhibition of Staphylococcus aureus by human semen. J. din. Path 4:360
    [Google Scholar]
  27. Harrison G. A. 1931; Spermine in human tissues. Biochem. J 25:1885
    [Google Scholar]
  28. Herbst E. J., Glinos E. B. 1955; An analysis of the putrescine requirement of Hemophilus parainfluenzae. J. biol. Chem 214:175
    [Google Scholar]
  29. Herbst E. J., Weaver R. H., Keister D. L. 1958; The gram reaction and cell composition: diamines and polyamines. Arch. Biochem. Biophys 75:171
    [Google Scholar]
  30. Herbst E. J., Whitherspoon B. H. 1960; Inhibition of spontaneous and induced swelling of mitochondria by spermine. Fed. Proc 19:138
    [Google Scholar]
  31. Kihara H., Snell E. E. 1957; Spermine and related polyamines as growth stimulants for Lactobacillus casei. Proc. nat. Acad. Sei., Wash 43:867
    [Google Scholar]
  32. Kosaki T., Ikoda T., Kotani Y., Nakagawa S., Saka T. 1958; A new phospholipid, malignolipin in human malignant tumors. Science 127:1176
    [Google Scholar]
  33. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring protein. Methods in Enzymology ed Colowick S. P., Kaplan N. O. 3451 New York: Academic Press, Inc;
    [Google Scholar]
  34. Luria S. E. 1960; Bacterial protoplasm—composition and organisation. The Bacteria ed Gunsalus I. C., Stanier R. Y. 127 New York: Academic Press Inc;
    [Google Scholar]
  35. Mager J. 1959; The stabilising effect of spermine and related polyamines and bacterial protoplasts. Biochem. biophys. Acta 36:529
    [Google Scholar]
  36. Martin W. H. Jun, Pelczar M. J. Jun, Hansen P. A. 1952; Putrescine as a growth requirement for Neisseria. Science 116:483
    [Google Scholar]
  37. Mitchell P. 1959; Biochemical cytology of micro-organisms. Annu. Rev. Microbiol 13:412
    [Google Scholar]
  38. Nason A., Evans H. J. 1953; Triphosphopyridine nucleotide-nitrate reductase in Neurospora. J. biol. Chem 202:655
    [Google Scholar]
  39. Razin S., Cohen A., Rozansky R. 1958; Antimycotic effect of spermine. Proc. Soc. exp. Biol., N.Y 99:459
    [Google Scholar]
  40. Razin S., Gery I., Bachrach U. 1958; Formation of /?-alanine from spermine and spermidine by Pseudomonas aeruginosa. Nature, Lond 181:700
    [Google Scholar]
  41. Razin S., Gery I., Bachrach U. 1959; The degradation of natural polyamines and diamines by bacteria. Biochem. J 71:551
    [Google Scholar]
  42. Razin S., Rozansky R. 1959; Mechanism of the antibacterial action of spermine. Arch. Biochem. Biophys 81:36
    [Google Scholar]
  43. Rosenthal S. M., Dubin D. T. 1960; Acetylation of polyamines by Staphylococcus. Fed. Proc 19:2
    [Google Scholar]
  44. Rosenthal S. M., Tabor C. W. 1956; The pharmacology of spermine and spermidine, distribution and excretion. J. Pharmacol 116:131
    [Google Scholar]
  45. Rozansky R., Bachrach U., Grossowicz N. 1954; Studies on the antibacterial action of spermine. J. gen. Microbiol 10:11
    [Google Scholar]
  46. Salton M. R. J., Horne R. W. 1951; Studies of the bacterial cell wall. 2. Methods of preparation and some properties of cell walls. Biochim. biophys. Acta 7:177
    [Google Scholar]
  47. Sneath P. H. A. 1955; Putrescine as an essential growth factor for a mutant of Aspergillus nidulans. Nature, Lond 175:818
    [Google Scholar]
  48. Tabor C. W. 1960; The stabilizing effect of spermine and related amines on mito-chondria and protoplasts. Biochem. biophys. Res. Comm 2:117
    [Google Scholar]
  49. Tabor H., Rosenthal S. M., Tabor C. W. 1957; The role of putrescine and methionine in the enzymatic synthesis of spermidine in Escherichia coli extracts. J. Amer. chem. Soc 79:2978
    [Google Scholar]
  50. Tabor H., Rosenthal S. M., Tabor C. W. 1958; The biosynthesis of spermidine and spermine from putrescine and methionine. J. biol. Chem 233:907
    [Google Scholar]
  51. Tabor H., Tabor C. W. 1960; Further purification of the enzymes concerned with the biosynthesis of spermidine in E. coli. Fed. Proc 19:6
    [Google Scholar]
  52. Traub A., Mager J., Grossowicz N. 1955; Studies on the nutrition of Pasteurella tularensis. J. Bad 70:60
    [Google Scholar]
  53. Weaver R. H., Herbst E. J. 1958; Metabolism of diamines and polyamines in microorganisms. J. biol. Chem 231:637
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-26-1-1
Loading
/content/journal/micro/10.1099/00221287-26-1-1
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error