1887

Abstract

SUMMARY: A critical examination of results obtained with several microculture techniques showed that considerable variations may occur in the formation of cell aggregations, localized in definite zones in the preparation. The cellular aggregation studied in the greatest detail was the clonal microcolony. Time-lapse photomicrography of developing microcolonies revealed that the arrangement of organisms follows an orderly pattern in both the smooth and rough phases. The genealogical history of the microcolony showed that cells are grouped by genealogical origin. The microcolonial configuration in both smooth and rough phases, at room temperature and 87°, consisted of closely packed arrays of organisms. At these temperatures, palisading appeared to be the primary movement through which the structure of the microcolonies was established. The usual palisading movement was of the sliding type, although buckling palisades were occasionally found in rough-phase cultures. At 44°, the microcolonial configuration in both smooth and rough phases did not invariably evolve through palisading. In these aberrant cases, the organisms appeared simply to push each other about, so that the final appearance was that of a loosely packed collection of organisms presenting as an irregular reticulum. Nevertheless, genealogical distribution remained orderly.

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/content/journal/micro/10.1099/00221287-25-3-353
1961-07-01
2024-04-25
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References

  1. Abercrombie M., Heaysman J. E. M. 1954; Observations on the social behaviour of cells in tissue culture. II. ‘Monolayering’ of fibroblasts. Exp. Cell Res 6:293
    [Google Scholar]
  2. Bayne-Jones S., Adolph E. F. 1932; Growth in size of microorganisms measured from motion pictures. III. Bacterium coli . J. cell. comp. Physiol 2:329
    [Google Scholar]
  3. Bisset K. A. 1938; The structure of ‘rough’ and ‘smooth’ colonies. J. Path. Bact 47:223
    [Google Scholar]
  4. Bisset K. A. 1950 The Cytology and Life-History of Bacteria p. 65 Baltimore: Williams and Wilkins;
    [Google Scholar]
  5. Dean A. C. R., Hinshelwood C. 1957; The formation of papillae on bacterial colonies. II. Proc. Roy. Soc. B 147:10
    [Google Scholar]
  6. Fleming A., Voureka A., Kramer I. R. H., Hughes W. H. 1950; The morphology and motility of Proteus vulgaris and other organisms cultured in the presence of penicillin. J. gen. Microbiol 4:257
    [Google Scholar]
  7. Graham-Smith G. 1910; The division and post-fission movements of bacilli when grown on solid media. Parasitology 3:17
    [Google Scholar]
  8. Hu K. 1936; Studien über den Entwicklungsmodus der Bakterien bei Einzellkultur auf Nährbodenfilm. III. Mitteilung. Beobachtungen über den Entwicklungsmodus der S- und R-Form der Bakterien. Jap. J. exp. Med 14:67
    [Google Scholar]
  9. Hughes W. H. 1953; The origin of the L-form variants in anaerobic cultures of Bacterium coli . J. gen. Microbiol 8:307
    [Google Scholar]
  10. Knaysi G. 1951 Elements of Bacterial Cytology, 2nd ed. p. 290 Ithaca, N.Y: Comstock Publishing Co;
    [Google Scholar]
  11. Lederberg J. 1956; Conjugal pairing in Escherichia coli . J. Bact 71:497
    [Google Scholar]
  12. Minck R., Ebel J.-P., Minck A. 1950; Le choix du fixateur en cytologie bactérienne. C.R. Soc. Biol., Paris 144:288
    [Google Scholar]
  13. Nutt M. M. 1927; The method of division of the rough and smooth type of colonies among the bacilli of the Salmonella group. J. Hyg., Camb 26:44
    [Google Scholar]
  14. Pearce T. W., Powell E. O. 1951; New techniques for the study of growing organisms. J. gen. Microbiol 5:91
    [Google Scholar]
  15. Pulvertaft R. J. V. 1952; Effects of antibiotics on growing cultures of Bacterium coli . J. Path. Bact 64:75
    [Google Scholar]
  16. Schneweis K.-E. 1959; Zur Mikromorphologie glatter und rauher Enterobacteriaceen-Kulturen. Zbl. Bakt 1 Abt. Orig 176:71
    [Google Scholar]
  17. Seal S. C. 1937; Rough and smooth cholera vibrios in relation to their mode of division and growth. Indian J. med. Res 24:991
    [Google Scholar]
  18. Topley and Wilson’s Principles of Bacteriology and Immunity 1955, 4th ed. Wilson G. S., Miles A. A. Ed p. 41 Baltimore: Williams and Wilkins;
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