1887

Abstract

SUMMARY: The development of tetanus intoxication in the frog is dependent on the environmental temperature being above about 15°. The incubation period and time to death become shorter as the environmental temperature is raised. Cooling below about 15° prevents both the fixation of the toxin and its action after fixation but does not increase the speed at which the toxin is destroyed or excreted. The absorption of the toxin after injection into the dorsal lymph sac is not prevented by cooling. It is possible to produce local tetanus in frogs by the intramuscular injection of tetanus toxin, but the dose needed for this is very critical. In most cases either generalized tetanus develops or there are no signs of intoxication. However, local tetanus can regularly be produced when the toxin is given intramuscularly to frogs partially protected from generalized tetanus by an injection of tetanus antitoxin given via the dorsal lymph sac. The local tetanus so produced does not progress to involve the opposite limb. The muscular spasm is abolished by cutting the motor nerve or by general anaesthesia. Tetanus toxin appears to act on the central nervous system of the frog in the same manner as in mammals. However, frog brain tissue does not neutralize tetanus toxin in low concentrations as does mammalian brain tissue, but concentrated tetanus toxoid gives immediate protection to frogs in the same way as it does in mammals.

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/content/journal/micro/10.1099/00221287-25-2-315
1961-06-01
2024-04-25
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References

  1. Baylis J. H., Mackintosh J., Morgan R. S., Wright G. P. 1952; The effect of sclerosis of the nerve trunk on the ascent of tetanus toxin in the sciatic nerve of rabbits and on the development of local tetanus. J. Path. Pact 64:33
    [Google Scholar]
  2. Brooks V. B., Curtis D. R., Eccles J. C. 1957; The action of tetanus toxin on the inhibition of motoneurones. J. Physiol 135:655
    [Google Scholar]
  3. Brunner C. 1894; Experimentelle und klinische Studien iiber den Kopftetanus. Beitr. klin. Chir 12:523
    [Google Scholar]
  4. Courmont J., Doyon M. 1893; Du tetanos de la grenouille et des conditions de temperature ambiante nécessaires a son apparition. C.B. Soc. Biol., Paris 9S 5:618
    [Google Scholar]
  5. Cowles R. B., Nelson N. B. 1947; Studies on thermal sedation in suppression of the symptoms of tetanus toxin. Proc. Soc. exp. Biol., N.Y 64:220
    [Google Scholar]
  6. Davies J. R., Morgan R. S., Wright E. A. 1955; The susceptibility of pigeons to tetanus toxin. J. Path. Bact 69:295
    [Google Scholar]
  7. Davies J. R., Wright E. A. 1955; The specific precocious protective action of tetanus toxoid. Brit. J. exp. Path 36:487
    [Google Scholar]
  8. Fildes P. 1931; . A System of Bacteriology in Relation to Medicine 3339 London: H.M. Stationery Office;
    [Google Scholar]
  9. Foxon G. E. H., Rowson K. E. K. 1956; The fate of Thorotrast (thorium dioxide) injected into the dorsal lymph sac of the frog, Rana temporaria . Quart. J. micr. Sci 97:47
    [Google Scholar]
  10. Friedemann U., Zuger B., Hollander A. 1939; Investigations on the pathogenesis of tetanus. J. Immunol 36:473
    [Google Scholar]
  11. Fröhlich A., Meyer H. H. 1916; Untersuchungen über den Tetanus. Arch. exp. Path. Pharmak 79:55
    [Google Scholar]
  12. Fulthorpe A. J. 1956; Absorption of tetanus toxin by brain tissue. J. Hyg., Camb 54:315
    [Google Scholar]
  13. Grasset E., Zutendyk A. 1931; Immunological studies in reptiles and their relation to aspects of immunity in higher animals. Publ. S. Afr. Inst. med. Res 4:377
    [Google Scholar]
  14. Gumprecht F. 1895; Versuch über die physiologischen Wirkungen des Tetanusgiftes im Organismus. Arch. ges. Physiol 59:105
    [Google Scholar]
  15. Herwick R. P., Weir E. P., Tatum A. L. 1936; Seasonal variation in susceptibility of animals to tetanus toxin. Proc. Soc. exp. Biol., N.Y 35:256
    [Google Scholar]
  16. Ipsen J. 1941 Contributions to the Theory of Biological Standardisation Part III Copenhagen: Munksgaard;
    [Google Scholar]
  17. Ipsen J. 1951; The effect of environmental temperature on the reaction of mice to tetanus toxin. J. Immunol 66:687
    [Google Scholar]
  18. Isayma S. 1924; Über die Geschwindigkeit des Flüssigkeitsaustausches zwischen Blut und Gewebe. Z. Biol 82:101
    [Google Scholar]
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