Inhibition of Enzyme Formation Following Infection of with phage T2r+ Free

Abstract

SUMMARY: The synthesis of aspartate carbamyl transferase (ureidosuccinic synthetase), of dihydroorotic dehydrogenase and of alkaline phosphatase by a uracil-requiring strain of when the organisms were suspended in a minimal medium lacking uracil, was followed subsequent to infection of the cells with bacteriophage T2r+ or with ghosts of the bacteriophage particles. The results were compared with the synthesis of these enzymes in uninfected cells. Following infection, the formation of all three enzymes was halted. Supplementation of the medium with casein hydrolysate and tryptophan or with 0·0l-Mg + 10 -spermine did not prevent the inhibition caused by bacteriophage infection.

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1961-06-01
2024-03-28
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References

  1. Adams M. H. 1950; Methods of study of bacterial viruses. Melh. med. Res 2:1
    [Google Scholar]
  2. Barner H. D., Cohen S. S. 1954; The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli . J. Bact 68:80
    [Google Scholar]
  3. Benzer S. 1953; Induced synthesis of enzymes in bacteria analyzed at the cellular level. Biochim. biophys. Acta 11:383
    [Google Scholar]
  4. Bessman M. J. 1959; Deoxyribonucleotide kinases in normal and virus infected Escherichia coli . J. biol. Chem 234:2735
    [Google Scholar]
  5. Bessman M. J., van Bibber M. J. 1959; A change in the properties of deoxyguanylate kinase of E. coli caused by viral infection. Biochem. biophys. res. Comm 1:101
    [Google Scholar]
  6. Burton K. 1955; The relation between the synthesis of deoxyribonucleic acid and the synthesis of protein in the multiplication of bacteriophage T2. Biochem. J 61:473
    [Google Scholar]
  7. Cohen S. S. 1948; The synthesis of bacterial viruses. I. The synthesis of nucleic acid and protein in Escherichia coli B infected with T2r+ bacteriophage. J. biol. Chem 174:281
    [Google Scholar]
  8. Delbrück M. 1940; The growth of bacteriophage and lysis of the host. J. gen. Physiol 23:643
    [Google Scholar]
  9. French R. C., Siminovitch L. 1955; The action of T2 bacteriophage ghosts on Escherichia coli B. Canad. J. Microbiol 1:757
    [Google Scholar]
  10. Gorini L., Maas W. 1957; The potential for the formation of a biosynthetic enzyme in Escherichia coli . Biochim. biophys. Acta 25:208
    [Google Scholar]
  11. Herriott R. M., Barlow J. L. 1957; The protein coats or ‘ghosts’ of coliphage T2. I. Preparation, assay and some chemical properties. J. gen. Physiol 40:809
    [Google Scholar]
  12. Hershey A. D., Dixon J. D., Chase M. 1953; Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition. J. gen. Physiol 36:777
    [Google Scholar]
  13. Hook A. E., Beard D., Taylor A. R., Sharp D. G., Beard J. W. 1946; Isolation and characterization of the T2 bacteriophage of Escherichia coli . J. biol. Chem 165:241
    [Google Scholar]
  14. Keck K., Mahler H. R., Fraser D. 1960; Synthesis of deoxycytidine 5′-phosphate deaminase in Escherichia coli infected by T2 bacteriophage. Arch. Biochem. Biophys 86:85
    [Google Scholar]
  15. Kornberg A., Zimmerman S. B., Kornberg S. R., Josse J. 1959; Enzymatic synthesis of deoxyribonucleic acid. VI. Influence of bacteriophage T2 on the synthetic pathway in host cells. Proc. nat. Acad. Sci., Wash 45:772
    [Google Scholar]
  16. Kozloff L. M., Knowlton K., Putnam F. W., Evans E. A. 1951; Biochemical studies of virus reproduction. V. The origin of bacteriophage nitrogen. J. biol. Chem 188:101
    [Google Scholar]
  17. Levin A. P., Magasanik B. 1961; The effect of purines on the formation of two enzymes involved in purine biosynthesis. J. biol. Chem 236:184
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Faar A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. J. biol. Chem 193:265
    [Google Scholar]
  19. Mager J. 1959; The stabilizing effect of spermine and related polyamines and protoplasts. Biochim. biophys. Acta 36:529
    [Google Scholar]
  20. McFall E., Pardee A. B., Stent G. S. 1958; The effects of radiophosphorous decay on some synthetic capacities of bacteria. Biochim. biophys. Acta 27:282
    [Google Scholar]
  21. McFall E., Magasanik B. 1960; Thymine starvation and enzyme synthesis. Biochim. biophys. Acta 45:610
    [Google Scholar]
  22. Monod J., Wollman E. 1947; L’inhibition de la croissance et de l’adaptation enzymatique chez les bactéries infectées par le bacteriophage. Ann. Inst. Pasteur 73:937
    [Google Scholar]
  23. Puck T. T., Lee H. H. 1954; Mechanism of cell wall penetration by viruses. I. An increase in host cell permeability induced by bacteriophage infection. J. exp. Med 99:481
    [Google Scholar]
  24. Puck T. T., Lee H. H. 1955; Mechanism of cell wall penetration by viruses. II. Demonstration of cyclic permeability change accompanying virus infection of Escherichia coli B. cells. J. exp. Med 101:151
    [Google Scholar]
  25. Riley M., Pardee A. B., Jacob F., Monod J. 1960; On the expression of a structural gene. J. mol. Biol 2:216
    [Google Scholar]
  26. Sher I. H., Mallette M. F. 1954; The adaptive nature of the formation of lysine de-carboxylase in Escherichia coli B. Arch. Biochem. Biophys 52:331
    [Google Scholar]
  27. Sommerville R., Ebisuzaki K., Greenberg G. R. 1959; Hydroxymethyldeoxycytidylate kinase formation after bacteriophage infection of Escherichia coli . Proc. nat. Acad. Sci., Wash 45:1240
    [Google Scholar]
  28. Torriani A. M. 1960; Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli . Biochim. biophys. Acta 38:460
    [Google Scholar]
  29. Vogel H. J. 1957; Repression and induction as control mechanisms of enzyme biogenesis: The ‘adaptive’ formation of acetylomithinase. In The Chemical Basis of Heridity p. 276 McElroy W. D., Glass B. Ed Baltimore, Md. U.S.A: The Johns Hopkins Press;
    [Google Scholar]
  30. Weed L. L., Cohen S. S. 1951; The utilization of host pyrimidines in the synthesis of bacterial viruses. J. biol. Chem 192:693
    [Google Scholar]
  31. Yates R. A., Pardee A. B. 1957; Control by uracil of formation of enzymes required for orotate synthesis. J. biol. Chem 227:677
    [Google Scholar]
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