1887

Abstract

SUMMARY: Microbial hydrolysis of triglycerides was observed when these were incubated anaerobically at 37° with sheep rumen contents. The extent of hydrolysis was variable, but was often considerable ( > 90 %) when linseed oil was used as substrate. The free fatty acids liberated Were analysed by gas chromatography and, as compared with the acids present initially in glyceride combination, they were less unsaturated because of microbial hydrogenation. Linolenic acid was particularly effectively hydrogenated. No synthesis of long-chain fatty acids took place during the incubations and, apart from the possibility that in some experiments a limited conversion of stearic acid to palmitic acid took place, there was no evidence of significant degradation of long-chain acids. Glycerol liberated during the hydrolysis was completely metabolized, in part to volatile fatty acids, largely propionic acid. No mono- or diglycerides were detected as intermediates in the lipolysis of triglycerides. Analysis of the contents of the rumen, abomasum and small intestine of each of two slaughtered sheep, one of which had previously been fed on a diet rich in linseed oil, showed that most of the total higher fatty acids present in each of these three portions of the alimentary tract was in the form of free acids. It is concluded that microbial lipolysis results in the pre-digestion of much of the lipids ingested by the sheep as part of its feed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-25-2-215
1961-06-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/25/2/mic-25-2-215.html?itemId=/content/journal/micro/10.1099/00221287-25-2-215&mimeType=html&fmt=ahah

References

  1. Barron E. J., Hanahan D. J. 1958; Observations on the silicic acid chromatography of the neutral lipids of rat liver, beef liver and yeast. J. biol. Chem 231:493
    [Google Scholar]
  2. Borgström B. 1954; Lipid separation methods. III. Separation of tri-, di-, 1-mono- and 2-monoglycerides. Acta physiol. scand 30:231
    [Google Scholar]
  3. Dawson R. M. C. 1959; Hydrolysis of lecithin and lysolecithin by rumen microorganisms of the sheep. Nature; Lond: 1831822
    [Google Scholar]
  4. Elsden S. R., Lewis D. 1953; The production of fatty acids by a Gram-negative coccus. Biochem. J 55:183
    [Google Scholar]
  5. Farquhar J. W., Insull W., Rosen P., Stoffel W., Ahrens E. H. 1959; The analysis of fatty acid mixtures by gas-liquid chromatography. Supplement (Number 8, Part II) to Nutr. Rev 17:
    [Google Scholar]
  6. Friedemann T. E. 1938; The identification and quantitative determination of volatile alcohols and acids. J. biol. Chem 123:161
    [Google Scholar]
  7. Garton G. A. 1959; Lipids in relation to rumen function. Proc. Nutr. Soc 18:112
    [Google Scholar]
  8. Garton G. A. 1960; Fatty acid composition of the lipids of pasture grasses. Nature; Lond: 187511
    [Google Scholar]
  9. Garton G. A., Hobson P. N., Lough A. K. 1958; Lipolysis in the rumen. Nature; Lond: 1821511
    [Google Scholar]
  10. Carton G. A., Lough A. K., Vioque E. 1959; The effect of sheep rumen contents on triglycerides in vitro . Biochem. J 73:46P
    [Google Scholar]
  11. Gray F. V., Pilgrim A. F., Rodda H. J., Weller R. A. 1952; Fermentation in the sheep. IV. The nature and origin of the volatile fatty acids in the rumen of the sheep. J. exp. Biol 29:57
    [Google Scholar]
  12. Hirsch J., Ahrens E. H. Jun 1958; The separation of complex lipid mixtures by the use of silicic acid chromatography. J. biol. Chem 233:311
    [Google Scholar]
  13. Hobson P. N., Mann S. O. 1961; The isolation and culture of glycerol-fermenting and lipolytic bacteria from the rumen of the sheep. J. gen. Microbiol 25:227
    [Google Scholar]
  14. Johns A. T. 1953; Fermentation of glycerol in the rumen of the sheep. N.Z. J. Sci. Tech 35A:262
    [Google Scholar]
  15. Korn E. D. 1960; The fatty acid and positional specificities of lipoprotein lipase. Abstr. Comms. 5th int. Conf. Biochem. Problems of Lipids p. 20
    [Google Scholar]
  16. Lambert M., Neish A. C. 1950; Estimation of glycerol. Canad. J. Res 28:83
    [Google Scholar]
  17. Reiser R. 1951; Hydrogenation of polyunsaturated fatty acids by the ruminant. Fed. Proc 10:236
    [Google Scholar]
  18. Reiser R., Reddy H. G. R. 1956; The hydrogenation of dietary unsaturated fatty acids by the ruminant. J. Amer. Oil Chem. Soc 33:155
    [Google Scholar]
  19. Shorland F. B., Weenink R. O., Johns A. T. 1955; Effect of the rumen on dietary fat. Nature; Lond: 1751129
    [Google Scholar]
  20. Shorland F. B., Weenink R. O., Johns A. T., McDonald I. R. C. 1957; The effect of sheep-rumen contents on unsaturated fatty acids. Biochem. J 67:328
    [Google Scholar]
  21. Weenink R. O. 1959; A note on the acetone-soluble lipids of forage grasses and clovers. N.Z. J. Sci 2:273
    [Google Scholar]
  22. Wright D. E. 1959; Hydrogenation of lipids by rumen protozoa. Nature; Lond: 184875
    [Google Scholar]
  23. Wright D. E. 1960; Hydrogenation of chloroplast lipids by rumen bacteria. Nature; Lond: 185546
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-25-2-215
Loading
/content/journal/micro/10.1099/00221287-25-2-215
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error